复方保肝饮料对CCl_(4)诱发小鼠急性化学性肝损伤的保护作用研究  

Protective Effect of Compound Liver-Protecting Beverage on Acute Chemical Liver Injury Induced by CCL_(4)in Mice

作　　者：邓婷 刘旻栩 张媛[2] 许友赤 陈彤[2] DENG Ting;LIU Minxu;ZHANG Yuan;XU Youchi;CHEN Tong(Fujian Leephick Pharmaceutical Industry Co.,Ltd.,Longyan 364000,China;Longyan University,Longyan 364000,China)

机构地区：[1]福建省力菲克药业有限公司,福建龙岩364000 [2]龙岩学院,福建龙岩364000

出　　处：《现代食品》2024年第21期178-181,共4页Modern Food

基　　金：龙岩经开区(高新区)奇迈科技创新基金项目(0000110105)。

摘　　要：目的:研究以葛根、绿豆等为原料制备的复方保肝饮料对化学性肝损伤的保护作用及其机制。方法:将小鼠随机分成空白对照组、模型组及复方保肝饮料低、中、高3个剂量组(3.75、7.50、15.00 g·kg^(-1)),四氯化碳(CCl_(4))植物油溶液腹腔注射建立小鼠急性肝损伤模型,末次给药后禁食18 h,测血清谷丙转氨酶(Alanine Aminotransferase,ALT)活性,肝组织还原型谷胱甘肽(Glutathione,GSH)、丙二醛(Malondialdehyde,MDA)、总超氧化物歧化酶(Total Superoxide Dismutase,T-SOD)水平和Bcl-2基因、TNF-α基因的表达水平,观察肝组织病理变化。结果:与模型组相比,各剂量组血清ALT水平及MDA含量显著降低,GSH含量、T-SOD活性和Bcl-2基因的表达量均显著升高,差异均有统计学意义(P<0.05或P<0.01),而TNF-α基因的表达量虽然有一定程度的降低,仅高剂量组差异具有统计学意义(P<0.05);各剂量组对肝组织病理变化有不同程度的缓解作用。结论:复方保肝饮料对急性化学性肝损伤具有一定辅助保护作用。Objective:Study the protective effect of compound hepatoprotective beverage prepared from kudzu root,mung bean and other raw materials on chemical liver injury and explore its mechanism.Method:Mice were randomly divided into blank control group,model group,and compound hepatoprotective beverage(3.75,7.50,15.00 g·kg^(-1))of low,medium and high dose.Acute liver injury model was established by intraperitoneal injection of carbon tetrachloride(CCl_(4))vegetable oil solution in mice.After the last administration,fasting for 18 hours was performed to measure serum alanine aminotransferase(ALT)activity,liver tissue glutathione(GSH),malondialdehyde(MDA),total superoxide dismutase(T-SOD)levels,and expression levels of Bcl-2 gene and TNF-αgene.Pathological changes in liver tissue were observed.Result:Compared with the model group,the serum ALT level and MDA content were significantly reduced in each dose group,while the GSH content,T-SOD activity,and Bcl-2 gene expression were significantly increased,and the differences were statistically significant(P<0.05 or P<0.01).Although the expression level of TNF-αgene was slightly reduced,only the high-dose group showed significant differences and statistical significance(P<0.05);each dose group has varying degrees of alleviating effects on pathological changes in liver tissue.Conclusion:Compound hepatoprotective beverage has a certain auxiliary protective effect on acute chemical liver injury.

关 键 词：保肝饮料 四氯化碳 肝损伤 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]