LC-MS/MS法测定动物源性食品中五氯酚残留量的关键控制点分析  

Analysis of Critical Control Points for the Determination of Pentachlorophenol Residues in Animal Derived Food by LC-MS/MS

作　　者：陈彦宏 毛新武 胡凌 苗磊 刘佳 钱振杰 钟玉心 王宇 CHEN Yanhong;MAO Xinwu;HU Ling;MIAO Lei;LIU Jia;QIAN Zhenjie;ZHONG Yuxin;WANG Yu(Guangzhou Institute for Food Inspection,Guangzhou 511400,China;Party School of the Guangdong Provincial Committee of CPC,Guangzhou 510000,China)

机构地区：[1]广州市食品检验所,广东广州511400 [2]中共广东省委党校,广东广州510000

出　　处：《现代食品》2024年第21期182-187,共6页Modern Food

基　　金：广州市市场监督管理局科技项目(2022kj36)。

摘　　要：本研究基于自下而上的不确定度评定方法,系统分析了液相色谱-串联质谱法测定动物源性食品中五氯酚残留量引入的不确定度来源和因素,并识别和量化了影响检测结果准确性的关键控制点。结果表明,样品溶液定容和配制系列标准工作溶液过程为引入相对标准不确定度的最大分量,二者贡献率占比74.3%。分析发现,使用15 mL具刻度尖底离心管对样品溶液定容和100μL移液器配制系列标准工作溶液是导致其不确定度分量贡献率较大的主要因素,为影响检测结果准确性的关键控制点。优化关键控制点后,可有效提高检测结果的准确性。本研究有助于提高LC-MS/MS法测定动物源性食品中五氯酚残留量的准确性,为分析质量控制提供新思路。In this study,the sources and factors introducing uncertainties in the determination of pentachlorophenol residues in animal derived food by liquid chromatography-tandem mass spectrometry were systematically analysed based on the bottom-up method,and the critical control points affecting the accuracy of detection results were identified and quantified.The results showed that the sample volume and the preparation of a series of standard working solutions were the largest components introducing relative standard uncertainties,which contributed 74.3%of the total.It was found that the use of 15 mL conical centrifuge tubes for sample volume setting and 100μL pipette for the preparation of standard working solutions were the main factors contributing to the large uncertainty components,which were the critical control points affecting the accuracy of the detection results.The optimization of the critical control points can effectively improve the accuracy of the results.This study can help to improve the accuracy of the determination of pentachlorophenol residues in animal derived food by LC-MS/MS and provides new ideas for analytical quality control.

关 键 词：关键控制点 测量不确定度 五氯酚 液相色谱-串联质谱法 动物源性食品 

分 类 号：O657.63[理学—分析化学]