基于斑马鱼实验模型评价八珍粉改善脾胃作用的研究  

Evaluation of the Effect of Eight-Treasure Powder on the Improvement of Spleen and Stomach Functions Based on Zebrafish Experimental Model

作　　者：陆雪娇 陈益润 刘晓茹 刘天煜 LU Xuejiao;CHEN Yirun;LIU Xiaoru;LIU Tianyu(Huajie Biotechnology(Hangzhou)Co.,Ltd.,Hangzhou 310000,China;Hangzhou Huajie Food Co.,Ltd.,Hangzhou 310000,China)

机构地区：[1]花姐生物科技(杭州)有限公司,浙江杭州310000 [2]杭州花姐食品有限公司,浙江杭州310000

出　　处：《现代食品》2024年第21期210-214,共5页Modern Food

摘　　要：目的:研究八珍粉对脾胃的改善作用。方法:动态随机选取3 dpf(受精后3 d)斑马鱼,以水溶方式给予三硝基苯磺酸构建胃肠黏膜损伤模型,建模成功后以水溶方式分别给予八珍粉和泼尼松,用阿尔新蓝染色,通过分析斑马鱼肠道杯状细胞数量评价八珍粉对胃肠黏膜损伤的修复功效;动态随机选取5 dpf(受精后5 d)斑马鱼,以水溶方式给予硫酸铝建立便秘模型,建模成功后以水溶方式给予八珍粉和多潘立酮,通过分析斑马鱼肠道标记强度评价八珍粉对便秘的改善功效。结果:1000、2000μg·mL^(-1)八珍粉对斑马鱼胃肠黏膜损伤具有显著的改善作用,且呈现剂量相关性;500、1000、2000μg·mL^(-1)八珍粉对斑马鱼便秘具有显著的改善作用,且现呈剂量相关性。结论:八珍粉对斑马鱼脾胃有一定的改善作用。Objective:To investigate the effects of eight-treasure powder on the improvement of spleen and stomach functions.Methods:3 dpf(3 days post-fertilization)zebrafish are randomly selected in a dynamic manner to construct a gastrointestinal mucosal injury model by giving tritrobenzene sulfonate in a water-soluble manner.After successful modeling,eight-treasure powder and prednisolone are given in water solution,respectively.The repair effect of eighttreasure powder on gastrointestinal mucosal injury is evaluated by analyzing the number of intestinal cup-cells in zebrafish stained with Alcian blue.5 dpf(5 days post-fertilization)zebrafish are randomly selected dynamically and given aluminum sulfate in water solution to establish a constipation model.After successful modeling,eight-treasure powder and domperidone are given in the way of water solution,and the improvement effect of eight-treasure powder on constipation is evaluated by analyzing the intestinal fluorescence intensity of the zebrafish.Result:1000,2000μg·mL^(-1)eight-treasure powder significantly improve the gastrointestinal mucosal injury of zebrafish,and show dose correlation.500,1000,2000μg·mL^(-1)eight-treasure powder has a significant effect on zebrafish constipation,and it is dose-dependent.Conclusion:Eight-treasure powder can improve the spleen and stomach of zebrafish to some extent.

关 键 词：八珍粉 斑马鱼 胃肠黏膜损伤 便秘 

分 类 号：R285[医药卫生—中药学]