比较尼莫地平两种给药途径对兔蛛网膜下腔出血后基底动脉Rho/ROCK信号通路的影响  

Comparison of the effects of two administration routes of nimodipine on the Rho/ROCK signaling path-way in the basilar artery after subarachnoid hemorrhage in rabbits

作　　者：陈金 丁浩 张松松[1] 施贤清[1] Chen Jin;Ding Hao;Zhang Songsong;Shi Xianqing(Intensive Care Unit,Guizhou Provincial People′s Hospital,Guiyang,Guizhou 550002,China;Intensive Care Unit,Guizhou Orthopedic Hospital,Guiyang,Guizhou 550007,China)

机构地区：[1]贵州省人民医院重症监护科,贵州贵阳550002 [2]贵州骨科医院重症监护科,贵州贵阳550007

出　　处：《中国药物与临床》2024年第23期1512-1515,共4页Chinese Remedies & Clinics

基　　金：贵州省卫生健康委员会科学技术基金项目(gzwkj2021-037)。

摘　　要：目的探究尼莫地平静脉给药和枕大池给药对蛛网膜下腔出血(SAH)新西兰大白兔后基底动脉Rho/RhoA相关蛋白激酶(ROCK)信号通路的影响。方法随机将32只新西兰大白兔分为4组,8只/组,分别为蛛网膜下腔出血(SAH)组、静脉尼莫地平(NV)干预组、假手术组、枕大池尼莫地平(ND)干预组。ND组、SAH组、NV组动物注入非抗凝的自体动脉血(1 ml/kg)制造SAH模型,假手术组注入37℃的0.9%氯化钠注射液0.5 ml/kg;30 min后,SAH组注入0.5 ml/kg 2%尼莫地平,NV组经兔耳缘静脉注入0.5 ml/kg 2%尼莫地平,SAH组和假手术组注入等量0.9%氯化钠注射液1 ml/kg。SAH后72 h进行神经功能评分,蛋白质印迹检测基底动脉中肌球蛋白轻链(MLC)、Rho相关激酶2(Rock2)、钙调蛋白(CAM)蛋白表达。结果假手术组比较,SAH组、ND组、NV组,ROCK2表达上调、MLC/CAM表达下降;与SAH组比较、ND组、NV组,ROCK2表达下降、MLC/CAM表达上调;ND组比较、NV组ROCK2表达增高、MLC、CAM表达下降。结论与静脉注入途径相比,枕大池注入尼莫地平对兔SAH后基底动脉血管Rho/ROCK信号通路传统影响更显著。Objective Nimodipine was administered to a New Zealand white rabbit with subarachnoid hemorrhage to investigate the effects of two different routes of administration on the posterior basilar artery Rho/ROCK signaling pathway.Methods A total of thirty-two New Zealand white rabbits were randomly divided into 4 groups(n=8):subarachnoid hemorrhage(SAH)group,intravenous nimodipine(NV)intervention group,Sham operation(Sham)group and nimodipine(ND)intervention group.Non anticoagulant autologous arterial blood(1 ml/kg)was injected into the ND group,SAH group,and NV group animals through the occipital cistern to replicate the SAH model.The sham group was injected with 0.9%sodium chloride injection(0.5 ml/kg)at 37℃through the occipital cistern.Thirty minutes later,the SAH group was injected with 0.5 ml/kg of 2%nifedipine via the occipi-tal cistern,the NV group was injected with 0.5 ml/kg of 2%nifedipine via the rabbit ear vein,and the SAH and sham groups were injected with an equal amount of 0.9%sodium chloride injection(1 ml/kg).72 hours after SAH,neurological function scoring was performed,the expression of myosin light chain(MLC),Rho related kinase 2(Rock2),and calmodulin(CAM)proteins in the basilar artery were detected by western blot.Results Compared with the sham group,the SAH group,ND group,and NV group showed upregulation of ROCK2 expression and decreased MLC/CAM expression.Compared with the SAH group,ROCK2 expression decreased and MLC/CAM ex-pression increased in ND group and NV group.Compared with the ND group,showed an in ROCK2 expression in-crease and MLC and CAM expression decreased in NV group.Conclusion Compared with intravenous injection,injection of nifedipine into the occipital cistern can more effectively affect the traditional Rho/ROCK signaling pathway in the basilar artery after SAH.

关 键 词：蛛网膜下腔出血 枕大池 注射 静脉内 尼莫地平 血管痉挛 颅内 

分 类 号：R965[医药卫生—药理学]