流式细胞仪与蛋白分析仪检测联合评估颅脑损伤预后的效能研究  

Study on the Efficacy of Combining Flow Cytometry and Protein Analyzer Detection in Evaluating the Prognosis of Traumatic Brain Injury

作　　者：徐川林 林秀 林恒杰 方小丽 XU Chuan-lin;LIN Xiu;LIN Heng-jie;FANG Xiao-li(Laboratory Department,Zhangzhou Hospital Affiliated to Fujian Medical University,Fujian Zhangzhou 363000)

机构地区：[1]福建医科大学附属漳州市医院检验科,福建漳州363000

出　　处：《中国医疗器械信息》2024年第24期23-25,共3页China Medical Device Information

摘　　要：目的:探讨流式细胞仪及蛋白分析仪联合测定血清炎性因子在颅脑创伤患者预后评估中的效能。方法:将2022年1月~2023年6月福建医科大学附属漳州市医院诊治的65例急性颅脑损伤患者作为观察对象,根据入院时格拉斯哥昏迷评分标准(GCS)结果分为重型损伤组、中型损伤组、轻型损伤组,通过流式细胞仪、蛋白分析仪测定入院后第1天血清炎性因子,分析测定指标水平与颅脑损伤程度相关性,通过ROC曲线分析两种仪器测定指标联合评估预后的效能。结果:入院后应用流式细胞仪、蛋白分析仪测定,入院前三组患者的血清炎性指标水平,显示重型损伤组炎性指标水平最高,组间差异存在统计学意义(P<0.05)。入院4周后根据格拉斯哥评分(GOS)评估,预后良好26例,预后不良39例,测定显示预后不良组血清炎性指标水平均高于预后良好组(P<0.05)。ROC曲线分析,流式细胞仪、蛋白分析仪联合测定血清炎性因子水平评估颅脑损伤预后不良的AUC为0.855,灵敏度91.8%,特异度68.5%,高于单一仪器测定指标的预测效能(P<0.05)。结论:流式细胞仪及蛋白分析仪测定血清炎性因子水平有助于判断颅脑损伤病情,且联合检测预测预后效能良好。Objective:To explore the efficacy of combining flow cytometry and protein analyzer in measuring serum inflammatory factors in prognostic evaluation of patients with traumatic brain injury.Methods:65 patients with acute traumatic brain injury treated at Zhangzhou Hospital Affiliated to Fujian Medical University from January 2022 to June 2023 were selected as the observation subjects.They were divided into severe injury group,moderate injury group,and mild injury group based on the Glasgow Coma Scale(GCS)results at admission.The serum inflammatory factors were measured on the first day after admission using flow cytometry and protein analyzer,and the correlation between the measured index levels and the degree of traumatic brain injury was analyzed,analyze the efficacy of combining two instrument measurement indicators to evaluate prognosis through ROC curve analysis.Results:After admission,flow cytometry and protein analyzer were used to measure the serum inflammatory index levels of the three groups of patients before admission.The severe injury group had the highest inflammatory index level,and there was a statistically significant difference between the groups(P<0.05).After 4 weeks of admission,according to the Glasgow Rating Scale(GOS)evaluation,26 cases had a good prognosis and 39 cases had a poor prognosis.The measurement showed that the serum inflammatory index levels in the poor prognosis group were higher than those in the good prognosis group(P<0.05).ROC curve analysis,combined with flow cytometry and protein analyzer to determine serum inflammatory factor levels,assessed poor prognosis of traumatic brain injury with AUC of 0.855,sensitivity of 91.8%,specificity of 68.5%,higher than the predictive efficacy of single instrument measurement indicators(P<0.05).Conclusion:The determination of serum inflammatory cytokine levels by flow cytometry and protein analyzer is helpful in determining the condition of traumatic brain injury,and the combined detection has good predictive efficacy for prognosis.

关 键 词：流式细胞仪 蛋白分析仪 炎性因子 颅脑创伤 预后 

分 类 号：R446.1[医药卫生—诊断学]