Xpert MTB/RIF与BD BACTEC MGIT960分枝杆菌全自动快速液体培养监测仪在辅助诊断结核分枝杆菌感染的价值  

The Value of Xpert MTB/RIF and BD BACTEC MGIT 960 Automatic Rapid Liquid Culture Monitor in Assisting the Diagnosis of Mycobacterium Tuberculosis Infection

作　　者：洪瑞真 HONG Rui-zhen(Clinical Laboratory,Xiamen Third Hospital,Fujian Xiamen 361000)

机构地区：[1]厦门市第三医院检验科,福建厦门361000

出　　处：《中国医疗器械信息》2024年第24期26-28,124,共4页China Medical Device Information

摘　　要：目的:探讨Xpert MTB/RIF与BD BACTEC MGIT960分枝杆菌全自动快速液体培养监测仪在辅助诊断结核分枝杆菌感染的价值。方法:回顾性选取500例本院2021年5月~2024年2月就诊的疑似结核分枝杆菌感染患者,分别采用Xpert MTB/RIF与BD BACTEC MGIT960分枝杆菌全自动快速液体培养。比较2种方法对诊断结核分枝杆菌感染的单独、联合检测价值,并比较不同用药时间、用药种类及耐药结果。结果:经临床培养结果显示,500例疑似结核分枝杆菌感染患者确诊384例,其中肺结核282例,肺外结核102例;经Xpert MTB/RIF检测确诊361例,经BD BACTEC MGIT960检测确诊355例,经联合诊断结果确诊378例;联合诊断灵敏度、准确度高于单独检测,漏诊低于单独检测(P<0.05);BD BACTEC MGIT960检测用药时间>3个月的利福平(含利福霉素类)耐药性病例数高于Xpert MTB/RIF(P<0.05)。结论:Xpert MTB/RIF与BD BACTEC MGIT960分枝杆菌全自动快速液体培养对于结核分枝杆菌感染都具有较高诊断价值,为临床早期筛查诊断、检测药敏提供参考依据,以制定相应干预方案。Objective:To investigate the value of Xpert MTB/RIF and BD BACTEC MGIT 960 automatic rapid liquid culture monitor in the diagnosis of mycobacterium tuberculosis infection.Methods:500 patients with suspected mycobacterium tuberculosis infection in our hospital from May 2021 to February 2024 were retrospectively selected,and Xpert MTB/RIF and BD BACTEC MGIT 960 were respectively cultured by automatic rapid liquid culture.The value of single and combined detection of the two methods in the diagnosis of mycobacterium tuberculosis infection was compared,and the results of different drug duration,drug types and drug resistance were compared.Results:The clinical culture results showed that 384 cases of 500 suspected mycobacterium tuberculosis infection were confirmed,including 282 cases of pulmonary tuberculosis and 102 cases of extrapulmonary tuberculosis.361 cases were confirmed by Xpert MTB/RIF test,355 cases by BD BACTEC MGIT 960 test,and 378 cases by combined diagnosis.The sensitivity and accuracy of combined diagnosis were higher than that of single detection,and the missed diagnosis was lower than that of single detection(P<0.05).The number of drug resistance cases of rifampicin(including rifamycin)detected by BD BACTEC MGIT 960 for>3 months was higher than that of Xpert MTB/RIF(P<0.05).Conclusion:Both Xpert MTB/RIF and BD BACTEC MGIT 960 automatic rapid liquid culture have high diagnostic value for mycobacterium tuberculosis infection,and provide reference for early clinical screening diagnosis and detection of drug susceptibility,so as to develop corresponding intervention programs.

关 键 词：结核分枝杆菌感染 Xpert MTB/RIF BD BACTEC MGIT960 利福平耐药性 

分 类 号：R446.1[医药卫生—诊断学]