全自动免疫组化染色系统在胸腔积液病理诊断中的应用与价值  

Application and Value of Fully Automatic Immunohistochemical Staining System in the Pathological Diagnosis of Pleural Effusion

作　　者：陈惠芹 连爱琼[1] CHEN Hui-qin;LIAN Ai-qiong(Department of Pathology,Xinglin Branch,First Affiliated Hospital of Xiamen University,Fujian Xiamen 361022)

机构地区：[1]厦门大学附属第一医院杏林分院病理科,福建厦门361022

出　　处：《中国医疗器械信息》2024年第24期50-52,共3页China Medical Device Information

摘　　要：目的:探讨全自动免疫组化染色系统在胸腔积液病理诊断中的应用与价值。方法:选择2023年1月~2023年12月到本院接受治疗的83例胸腔积液患者作为研究对象。对本研究所有的患者进行全自动免疫组化染色系统检测,同时进行常规细胞学涂片检测,对比这两种检测方法的诊断价值。结果:全自动免疫组化染色系统检测较常规细胞学涂片表现出更高的阳性率(P<0.05);全自动免疫组化染色系统可以比较准确地对恶性胸腔积液的类型进行诊断,其准确率达到了96.39%。经过组合性抗体检测发现,间皮病变呈现Calretinin、WT-1、CK5/6、Desmin指标高表达,而肺腺癌则呈现CK7、NaspinA、SPA、Ki67、TTF-1、CEA指标高表达,表明全自动免疫组化染色系统的检测效果对比较为传统的细胞涂片检测会更高,两组之间具有统计学差异(P<0.05)。结论:全自动免疫组化染色系统在胸腔积液病理诊断中具有病理鉴别诊断的效果。Objective:Exploring the application and value of fully automated immunohistochemistry staining system in the pathological diagnosis of pleural effusion.Methods:83 pleural effusion patients treated in our hospital from January 2023 to December 2023 were selected as the study subjects.Perform a fully automated immunohistochemical staining system on all patients in this study,as well as routine cytological smear testing,to compare the diagnostic value of these two detection methods.Results:The positive rate of routine cytology smear detection was lower than that of fully automated immunohistochemistry staining system detection(P<0.05);The accuracy of the fully automated immunohistochemical staining system in detecting and diagnosing malignant pleural effusion was as high as 96.39%.After combination antibody testing,it was found that mesothelial lesions showed high expression of Calretin,WT-1,CK5/6,and Desmin indicators,while lung adenocarcinoma showed high expression of CK7,NaspinA,SPA,Ki67,TTF-1,and CEA indicators.The results showed that the automatic immunohistochemical staining system detection was significantly higher than conventional cell smear detection,and the difference between the two groups was statistically significant(P<0.05).Conclusion:The fully automatic immunohistochemical staining system has the effect of pathological differential diagnosis in the pathological diagnosis of pleural effusion.

关 键 词：细胞块切片 免疫组化 染色 应用价值 

分 类 号：R446.8[医药卫生—诊断学]