不同危重程度社区获得性肺炎患儿肺部微生物特征分析  

Microbial characteristics analysis of the lungs in children with community-acquired pneumonia of different severity levels

作　　者：吴勇 潘秀霞 秦华 刘运军 朱艳 王思佳 梁勇华 曾荣 吴倩 WU Yong;PAN Xiuxia;QIN Hua;LIU Yunjun;ZHU Yan;WANG Sijia;LIANG Yonghua;ZENG Rong;WU Qian(Department of Pediatrics,Jingmen People’s Hospital,Jingmen 448000,Hubei,China)

机构地区：[1]荆门市人民医院儿科,湖北荆门448000

出　　处：《中国现代医生》2024年第36期22-27,共6页China Modern Doctor

基　　金：湖北省荆门市科技计划一般项目(2023YFYB037)。

摘　　要：目的研究不同危重程度社区获得性肺炎(community-acquired pneumonia,CAP)患儿肺部微生物组学的特征,从而为肺炎患儿的精准诊断及抗生素治疗提供依据,并且为肺部微生态在肺炎患儿的诊断与治疗中的作用提供新策略和新视野。方法收集2023年1月至12月在荆门市人民医院儿科住院的64例不同危重程度CAP患儿的支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF),将患儿分为重症肺炎组(n=34)和普通肺炎组(n=30)。通过BALF的宏基因组测序获得不同危重程度CAP患儿肺部的微生物组学信息,对得出的两组样本的菌群生物信息数据进行菌群结构多样性分析、物种分类分析及差异性分析。结果Alpha多样性分析显示,两组患儿BALF的Chao1指数、ACE指数、Shannon指数、Simpson指数比较,差异均有统计学意义(P<0.05)。Beta多样性的主坐标分析(principal coordinate analysis,PCoA)显示,两组患儿BALF的微生态菌群组成相异性比较,差异有统计学意义(F=4.221,P=0.005)。经物种分类分析发现,在属水平,重症肺炎组患儿BALF样本中以支原体属为主,其次为链球菌属,第三为嗜血杆菌属,普通肺炎组患儿BALF样本中以链球菌属为主,其次为支原体属,第三为嗜血杆菌属。两组患儿的微生物种群在属水平层面排名前20位物种中丰度差异有统计学意义(P<0.05)的是支原体属、链球菌属、红球菌属、奈瑟菌属、普雷沃菌属、棒状杆菌属、假单胞菌属。物种差异性分析显示,在属水平,有47个物种有差异(P<0.05)。结论不同危重程度CAP患儿的肺部微生物菌群丰度、多样性及微生物组结构与组成存在差异性,不同危重程度CAP患儿间优势菌群有所不同,本研究丰富了CAP患儿的肺部微生物组学数据。Objective To study microbial characteristics of pulmonary in children with community-acquired pneumonia(CAP)of different severity,in order to provide a basis for accurate diagnosis and antibiotic treatment of pneumonia children,and provide new strategies and perspectives for the diagnosis and treatment of pulmonary microbiota in pneumonia children.Methods Bronchoalveolar lavage fluid(BALF)from 64 children with CAP of different severity hospitalized in Department of Pediatrics,Jingmen People’s Hospital were collected from January to December 2023,the children were divided into severe pneumonia group(n=34)and common pneumonia group(n=30).Microbiome information of the lungs of children with CAP of different severity were obtained through metagenomic sequencing of BALF,microbial structure diversity analysis,species classification analysis,and differential analysis on the microbial bioinformatics data of two groups of samples obtained were performed.Results Alpha diversity analysis showed that there were statistically significant differences(P<0.05)in the Chao1 index,ACE index,Shannon index,and Simpson index between two groups.The principal coordinate analysis(PCoA)of Beta diversity showed a statistically significant difference in the composition of microbial communities between two groups(F=4.221,P=0.005).Through species classification analysis,it was found that at the genus level,mycoplasma was the main genus in the BALF samples of severe pneumonia group,followed by Streptococcus and Haemophilus,Streptococcus was the main genus in the BALF samples of common pneumonia group,followed by Mycoplasma and Haemophilus.Children of two groups showed statistically significant differences in microbial abundance among the top 20 species at the genus level(P<0.05),including Mycoplasma,Streptococcus,Rhodococcus,Neisseria,Prevotella,Corynebacterium,and Pseudomonas.Species diversity analysis showed that at the genus level,there were 47 species with differences(P<0.05).Conclusion There are differences in the abundance,diversity,st

关 键 词：支气管肺泡灌洗液 宏基因组测序 社区获得性肺炎 肺部微生态 

分 类 号：R725.6[医药卫生—儿科]