基于HPLC-QAMS多指标成分联合OPLS-DA及加权TOPSIS模型的威灵仙药材质量评价  

Quality evaluation of Clematidis radix et rhizoma medicinal materials based on HPLC-QAMS multi-components quantification combined with OPLS-DA and weighted TOPSIS method

作　　者：冯晓川 张静 徐延昭[4] 张蕊 FENG Xiaochuan;ZHANG Jing;XU Yanzhao;ZHANG Rui(Department of Chinese Pharmacy,Beijing Jishuitan Hospital,Capital Medical University,Beijing 100035,China;The Fourth Clinical Medical College of Peking university,Beijing 100035,China;National Center for Orthopaedics,Beijing 100035,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京积水潭医院中药房,北京100035 [2]北京大学第四临床医学院,北京100035 [3]国家骨科医学中心,北京100035 [4]中国食品药品检定研究院,北京100050

出　　处：《药学前沿》2024年第12期593-604,共12页China Pharmacist

基　　金：全国中药特色技术传承人才培训项目(国中医药人教函〔2023〕96号);北京市医院管理中心“青苗”计划专项经费资助项目(QML20230407);北京市属医院科研培育项目(PZ2021005);北京积水潭医院“学科骨干”计划专项经费资助项目(XKGG202214)。

摘　　要：目的建立不同产地威灵仙中9个成分含量同步检测方法,筛选影响其质量的差异标志物,对其质量差异性进行评价。方法对8省16个批次威灵仙样品进行回流提取,提取物采用HPLC法检测;采用化学识别模式和加权TOPSIS法建立威灵仙质量优劣评价模型,对其质量差异性进行综合评价。结果灵仙新苷、虎掌草皂甙D、威灵仙皂甙C、虎掌草皂苷B、常春藤皂苷元、齐墩果酸、3,5,6,7,8,3',4'-七甲氧基黄酮、橙皮素和芒柄花素分别在1.27~31.75、4.48~112.00、7.35~183.75、3.69~92.25、6.16~154.00、20.95~523.75、0.58~14.50、0.39~9.75和0.26~6.50µg/mL范围内线性关系良好(r>0.9990),平均加样回收率为96.91%~100.12%,RSD为0.71%~1.53%(n=9);16批样品聚为3类;齐墩果酸、威灵仙皂甙C、灵仙新苷和常春藤皂苷元可能是影响威灵仙产品质量主要潜在标志物;加权TOPSIS法分析结果显示16批威灵仙质量评价贴近度在0.0978~0.8182之间,其中S13最大(0.8182)。结论建立的威灵仙中9种成分定量分析方法,操作简便、结果准确;化学计量学及加权TOPSIS法可用于评价其质量差异性。Objective To establish a method for simultaneous detection of 9 components in Clematidis radix et rhizoma from different producing areas,screen the differential markers affecting their quality,and to evaluate its quality difference.Methods Reflux extraction was performed on 16 batches of Clematidis radix et rhizoma from 8 provinces,and the extracts were detected by HPLC.The chemical identification model and weighted TOPSIS method were used to establish the quality evaluation model of Clematidis radix et rhizoma,and the quality difference was comprehensively evaluated.Results Clematichinenoside AR,huzhangoside D,clematichinenoside C,huzhangoside B,hederagenin,oleanolic acid,3,5,6,7,8,3',4'-heptamethoxyf lavone,hesperetin and formononetin showed good linear relationships within the ranges of 1.27-31.75,4.48-112.00,7.35-183.75,3.69-92.25,6.16-154.00,20.95-523.75,0.58-14.50,0.39-9.75 and 0.26-6.50µg/mL(r>0.9990),respectively.The average recovery rate was 96.91%-100.12%,and the RSD was 0.71%-1.53%(n=9).16 batches of samples were grouped into 3 categories,and oleanolic acid,clematichinenoside C,clematichinenoside AR and hederagenin might be the main potential markers affecting the quality of Clematidis radix et rhizoma.The analysis results of the weighted TOPSIS method revealed that the closeness for evaluating the quality of 16 batches of Clematidis radix et rhizoma ranged from 0.0978 to 0.8182,with S13 achieving the highest value of 0.8182.Conclusion The method for quantitative analysis of 9 components in Clematidis radix et rhizoma is simple and accurate.Chemometrics and weighted TOPSIS method can be used to evaluate the quality difference.

关 键 词：威灵仙 高效液相色谱法 化学计量学 主成分分析 正交偏最小二乘判别分析法 加权TOPSIS法 质量评价 

分 类 号：R284.1[医药卫生—中药学]