山楂酸对大鼠心肌缺血再灌注损伤HMGB1、IL-1β、IL-6表达及GSH-Px活性的影响  

Effects of Maslinic Acid on the Levels of HMGB1,IL-1β,IL-6 and GSH-Px Activity in Rats with Myocardial Ischemia-Reperfusion Injury

作　　者：张倩 王勇 ZHANG Qian;WANG Yong(The Central Hospital of Enshi Tujia and Miao Autonomous Perfecture/Enshi Clinical College of Wuhan University,Enshi 445000,China)

机构地区：[1]恩施土家族苗族自治州中心医院/武汉大学恩施临床学院,湖北恩施445000

出　　处：《西部中医药》2024年第12期7-10,共4页Western Journal of Traditional Chinese Medicine

基　　金：湖北省技术创新专项(2016ACA153)。

摘　　要：目的:观察山楂酸(maslinic acid,MA)对大鼠心肌缺血再灌注(ischemia/reperfusion,I/R)损伤高迁移率族蛋白1(high mobility group protein 1,HMGB1)、白细胞介素1β(interleukin-1β,IL-1β)、IL-6水平及谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性的影响。方法:将48只SD大鼠随机分成4组,即假手术组(Sham)、模型组(I/R)、MA低剂量组(10 mg/kg,MA-Low)、MA高剂量组(30 mg/kg,MA-High),每组12只。MA组造模前连续1周灌胃给药,通过结扎大鼠左冠状动脉前降支(left anterior descending,LAD)30 min再灌注2 h建立大鼠心肌I/R模型。取大鼠心肌缺血组织,TTC染色法测定心肌梗死面积,匀浆后采用比色法测定GSH-Px活性,ELISA检测IL-1β的含量,Western Blot和免疫荧光分别检测缺血心肌组织中HMGB1及IL-6蛋白的表达情况。结果:与I/R组比较,MA-Low组及MA-High组心肌梗死面积减小,HMGB1、IL-1β、IL-6蛋白水平降低,GSH-Px活性升高,其中MA-High组差异更显著(P<0.05)。结论:MA对缺血再灌注心肌具有保护作用,其机制可能是通过抑制HMGB1及炎性因子IL-1β、IL-6的表达,增强GSH-Px活性来实现的。Objective:To observe the effects of maslinic acid(MA)on the levels of HMGB1,IL-1β,IL-6 and GSH-Px activity in myocardial ischemia/reperfusion(I/R)rats.Methods:A total of 48 SD rats were randomized into four groups:sham operation group(Sham),the model group(I/R),low dose(10 mg/kg,MA-Low)group of MA,and high dose(30 mg/kg,MA-High)group of MA,with 12 in each group.MA group accepted intragastric administration of medicine for one week before modeling,rat models with myocardial I/R injury were established by left anterior descending(LAD)ligation for 30 min and then reperfusion for two hours.TTC staining was used to measure the area of myocardial infarction after taking myocardial ischemic tissue,the activity of GSH-Px was determined by colorimetric method after homogenizing the tissue,the contents of IL-1βwere detected by ELISA,the expressions of HMGB1 and IL-6 protein in the ischemic myocardial tissue were measured by Western Blot and immunofluorescence respectively.Results:Compared to I/R group,the area of myocardial infarction was reduced in MA-Low group and MA-high group,the levels of HMGB1,IL-1βand IL-6 protein lowered,the activity of GSH-Px was increased,among them,the difference was significant in MA-High group(P<0.05).Conclusion:MA has the protective effects on I/R myocardium,and its mechanism may be realized by inhibiting the expres-sions of HMGB1,IL-1βand IL-6,and enhancing the activity of GSH-Px.

关 键 词：心肌缺血再灌注损伤 山楂酸 高迁移率族蛋白1 白细胞介素 谷胱甘肽过氧化物酶 大鼠 

分 类 号：R256.21[医药卫生—中医内科学]