猕猴桃溃疡病生防菌群的构建及其生防效应  

Construction and Control Effect of Biocontrol Flora of Kiwifruit Bacterial Canker

作　　者：尹志诚 顾佳颖 李南羿 张昕[1] Yin Zhicheng;Gu Jiaying;Li Nanyi;Zhang Xin(Zhejiang Agriculture and Forestry University,Hanzhou 311300)

机构地区：[1]浙江农林大学林业与生物技术学院,杭州311300

出　　处：《林业科学》2024年第12期101-110,共10页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(31970093)。

摘　　要：【目的】从猕猴桃本体分离获得生防细菌构建生防菌群,明确浙江临安猕猴桃溃疡病病原菌的类群,探究生防菌群的生防效应,为猕猴桃溃疡病的防治提供新思路。【方法】从浙江临安猕猴桃种植区分离溃疡病菌并鉴定;基于健康、发病猕猴桃枝条内生菌的宏基因组测序比较和R语言分析,以该病菌为靶标分离生防细菌,构建生防菌群;通过离体共培养和活体接种试验,验证菌群的生防效应。【结果】1)浙江临安的猕猴桃溃疡病由丁香假单胞菌猕猴桃致病变种HY-05引起。枝条内生菌的宏基因组测序结果表明,与发病的猕猴桃植株相比,健康的猕猴桃枝条内部细菌群落之间更易形成积极的互作关系。2)基于R语言分析,从猕猴桃健康植株分离获得病原菌HY-05的拮抗菌株红树林动球菌ZC-3和蒙氏肠球菌ZC-5,以及ZC-3的益生菌嗜根寡养单胞菌ZC-6,构建成生防菌群。3)离体共培养试验结果表明,各供试菌株均能抑制菌株HY-05的生长,其中以ZC-3、ZC-5和ZC-6构建的生防菌群效果最佳,抑制率达100%;植株活体试验中,添加各生防菌组合的处理均可不同程度抑制猕猴桃溃疡病的发生,其中以ZC-3、ZC-5和ZC-6构建的生防菌群处理效果最佳,防治效果达56.00%,与发病对照相比差异极显著(P<0.01)。【结论】发生自浙江临安的猕猴桃溃疡病由丁香假单胞菌猕猴桃致病变种引起,从猕猴桃植株内分离获得的生防菌株构建的生防菌群经离体和活体实验证实具有实际生防效应。【Objective】In this study,biocontrol bacteria were isolated from kiwifruit and used for construction of biocontrol bacterial community.The pathogen of kiwifruit bacterial canker was identified in Lin'an,Zhejiang Province.This study aims to explore the biocontrol effect of the bacterial community on the pathogen of kiwifruit bacterial canker,so as to provide new insights for the prevention and control of kiwifruit bacterial canker.【Method】The pathogenic bacteria causing canker disease of kiwifruit were isolated from kiwifruit planting areas in Lin'an,Zhejiang Province and identified.Based on the comparison and analysis of the metagenomes of endophytic bacteria in healthy and diseased kiwifruit branches using R language,antagonistic bacteria against the pathogenic bacteria were isolated,and a biocontrol bacterial community was constructed.The biocontrol effect of the bacterial community on the pathogenic bacteria was validated through in vitro co-cultivation and in vivo inoculation experiments.【Result】1)Kiwifruit bacterial canker in Lin'an,Zhejiang Province was caused by Pseudomonas syringae pv.actinidiae HY-05.The metagenomic sequencing results of endophytic bacteria in kiwifruit branches showed that positive interactions were more likely to form among the bacterial communities in healthy kiwifruit branches compared to diseased ones.2)Based on R language analysis,antagonistic strains,Kineococcus mangrovi ZC-3 and Enterococcus mundtii ZC-5,against the pathogenic strain HY-05,as well as a probiotic strain,Stenotrophomonas rhizophila ZC-6,were isolated from healthy kiwifruit plants,and a biocontrol bacterial community was constructed.3)In the in vitro co-cultivation experiment,all tested strains inhibited the growth of HY-05,with the biocontrol bacterial community constructed by ZC-3,ZC-5,and ZC-6 showing the best inhibitory effect,with a suppression rate of 100%.In the in vivo plant experiment,treatments with different biocontrol bacterial combinations were able to inhibit the occurrence of kiwifruit bact

关 键 词：猕猴桃溃疡病 宏基因组测序 R语言 生防菌 菌群构建 生防效应 

分 类 号：S718.43[农业科学—林学]