接种丛枝菌根真菌促进磷高效基因型大豆生长和磷吸收  

Inoculating arbuscular mycorrhizal fungi promotes the growth and phosphorus uptake of phosphorus-efficient soybean genotype

作　　者：黄活志 刘洋[1] 陈阿 陈康 王秀荣[1] HUANG Huo-zhi;LIU Yang†;CHEN A;CHEN Kang;WANG Xiu-rong(State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Root Biology Center,South China Agricultural University,Guangzhou,Guangdong 510642,China)

机构地区：[1]华南农业大学亚热带农业生物资源保护与利用国家重点实验室/华南农业大学根系生物学研究中心,广东广州510642

出　　处：《植物营养与肥料学报》2024年第12期2354-2365,共12页Journal of Plant Nutrition and Fertilizers

基　　金：国家重点研发计划项目(2021YFF1000500)。

摘　　要：【目的】探究供磷和接种丛枝菌根真菌(arbuscular mycorrhizal fungus,AMF)对不同磷效率基因型大豆生长、磷吸收以及菌根诱导的磷转运蛋白和蔗糖转运蛋白基因表达的影响,分析AMF与磷效率的关系,为接种AMF提高大豆磷效率提供理论依据。【方法】以磷高效大豆巴西十号(BX10)和磷低效大豆本地2号(BD2)为材料进行盆栽试验,设置低磷和高磷两个磷水平,外源接种Rhizophagus irregularis(Ri)和Rhizophagus custos(Rc)两种AMF处理,以不接种AMF为对照(NM)。处理6周后,取地上部和根部样品,测定大豆植株干重和吸磷量、根冠比、菌根侵染率、根系性状,以及菌根诱导的磷转运蛋白和蔗糖转运蛋白基因的表达量。【结果】与低磷相比,高磷显著增加了大豆地上部和根部干重及吸磷量,降低了两个基因型大豆的菌根生长反应(MGR)和菌根磷反应(MPR)。低磷条件下,Ri、Rc处理显著增加了两个基因型大豆的地上部和根部干重及吸磷量,增加了磷高效基因型BX10的总根长、根表面积及根体积,相应地,两个基因型大豆负责菌根途径磷吸收的磷转运蛋白基因GmPT9的表达显著上调,磷低效基因型BD2中菌根诱导表达的GmPT8的表达量也显著上调,而主要负责根系途径磷吸收的GmPT4的表达量在接种与不接种AMF处理之间没有显著差异。在高、低磷条件下,不接种AMF的BX10大豆根部干重和根冠比都显著高于BD2;在高磷条件下,BX10大豆根平均直径显著高于BD2。在低磷接种AMF条件下,BX10大豆总根长、根表面积和根体积都显著高于BD2;在高磷接种AMF条件下,BX10大豆根表面积和根体积也显著高于BD2。接种Ri处理磷高效基因型大豆BX10地上部干重、根部干重和吸磷量均显著高于BD2,而低磷接种Ri处理的BD2根系中GmPT8和GmPT9及GmSWEET6的表达量均显著高于BX10。【结论】高磷显著促进了两个不同磷效率基因型大豆的生长和吸磷量,但低磷条件�【Objectives】We investigated the effects of phosphorus(P)supply level and inoculation of arbuscular mycorrhizal fungus(AMF)strains on the growth,phosphorus(P)uptake,and the expression of mycorrhiza-induced phosphate transporter and sucrose transporter genes in soybean genotypes with different P efficiency,as well as the relationship of AMF and P efficiency.【Methods】A pot experiment was conducted using P-efficient soybean genotype Baxi 10(BX10)and P-inefficient genotype Bendi No.2(BD2)as experimental materials.The treatment was composed of two P supply levels(low P,and high P),and exogenous inoculation of two AMF strains,Rhizophagus irregularis(Ri)or Rhizophagus custos(Rc),taking no AMF(NM)inoculation as the controls.After 6 weeks of treatment,the plant dry weight,P uptake,root-to-shoot ratio,mycorrhizal infection rate,root traits,and the expression of mycorrhiza-induced phosphate transporter genes and sucrose transporter gene were analyzed.【Results】Compared with low P,high P significantly increased the shoot and root dry weights as well as P uptake in soybean,reduced the mycorrhizal growth response(MGR)and mycorrhizal P response(MPR)of both genotypes of soybean.Under low P condition,both Ri and Rc significantly increased the shoot and root dry weights and P uptake of both the soybean genotypes,increased the total root length,root surface area,and root volume of the P-efficient genotype BX10.The expression of the phosphate transporter gene GmPT9,which is primarily responsible for P uptake via the mycorrhizal pathway,was significantly upregulated by AMF inoculation in both soybean genotypes as well.The expression of mycorrhiza-induced GmPT8 was also significantly upregulated in P-inefficient genotype BD2.However,there was no significant difference in the expression of GmPT4,which is primarily responsible for P uptake via root pathway,between the treatments with and without AMF inoculation.The root dry weight and root-to-shoot ratio of BX10 were significantly higher than those of BD2 under both low P and

关 键 词：丛枝菌根真菌 大豆基因型 菌根生长反应 菌根磷反应 GmPT9 GmPT4 

分 类 号：S565.1[农业科学—作物学]