响应面法优化黑根霉EPS分批发酵的温度分段控制工艺  

Two-stage temperature controlling strategy for batch fermentation process to optimize extraction of exopolysaccharides from Rhizopus nigricans by response surface methodology

作　　者：秦国正 张明芮[1] 李贵文 西传虎 方雪 王国栋 QIN Guozheng;ZHANG Mingrui;LI Guiwen;XI Chuanhu;FANG Xue;WANG Guodong(School of Pharmacy,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002 [2]皖南医学院安徽省多糖药物工程技术研究中心,安徽芜湖241002 [3]皖南医学院活性生物大分子研究安徽省重点实验室,安徽芜湖241002

出　　处：《皖南医学院学报》2024年第6期522-525,共4页Journal of Wannan Medical College

基　　金：安徽省自然科学基金项目(1908085MH248);芜湖市科技计划项目(2022cg20);皖南医学院校重点项目科研基金(WK2021Z19);安徽省大学生创新创业训练计划项目(S202310368078)。

摘　　要：目的:优化黑根霉胞外多糖(EPS)发酵条件,以获得黑根霉EPS发酵最优工艺,为后续药理学研究和药物开发提供材料保障。方法:响应面法(RSM)考察前段发酵时间(变温时间)、后段发酵温度和发酵时间3个因素对EPS产量的影响,预测EPS分批发酵最优工艺。结果:RSM结果显示,各因素按照对响应值的影响顺序为:发酵时间>后段发酵温度>前段发酵时间。发酵时间(P<0.001)、后段发酵温度(P=0.015)对EPS产量的影响显著。预测EPS发酵最优工艺为:前段发酵时间58.018 h,后段发酵温度26.984℃,发酵时间143.193 h,EPS产量为0.891 g/L。为验证工艺可靠性,结合设备条件和控制能力,将验证工艺条件优化设置为:前段发酵时间58 h,后段发酵温度27℃,发酵时间143 h。经验证,EPS产量为0.889 g/L。结论:通过RSM优化工艺,可以获得黑根霉EPS发酵最优工艺,实验重复性好,是一个稳健、可控和高产的工艺技术方案,可为后续药理学研究和药物开发提供材料保障。Objective:To optimize the fermentation process of extracting exopolysaccharides(EPS)from Rhizopus nigricans in order to obtain the optimal fermentation process for EPS for the evidence support in following pharmacological research and drug development.Methods:Response surface methodology(RSM)was used to investigate the effects of pre-fermentation duration(the optimal time for temperature change),post-fermentation temperature and fermentation duration on the yield of EPS from Rhizopus nigricans to eventually determine the optimal batch fermentation process for EPS extraction.Results:RSM analysis showed that the factors influencing the response value was associated with the effectivity by fermentation duration>post-fermentation temperature>pre-fermentation duration.The factors producing significant impact on EPS production were associated with fermentation time(P<0.001)and post fermentation temperature(P=0.015).The optimal fermentation condition was 58.018 h for the process of pre-fermentation duration,26.984℃for post-fermentation temperature,and 143.193 h for fermentation duration.Under such a condition,the maximumly predicted yield of EPS was 0.891 g/L.Based on the equipment conditions and control capabilities,we had optimized the process as 58 h for pre-fermentation duration,27℃for post-fermentation temperature,and 143 h for fermentation duration.After verification of the process as described above,the yield of EPS was obtained by 0.889 g/L.Conclusion:RSM may be used to optimize EPS fermentation process for Rhizopus nigricans.The process optimized in this study can be a support in following pharmacological research and drug development because it is reproductive,controllable and high-yielding.

关 键 词：黑根霉 胞外多糖 分批发酵 响应面法 

分 类 号：R285[医药卫生—中药学] R931.4[医药卫生—中医学]