金钗石斛总生物碱对大鼠后发性白内障的影响  

Effects of Dendrobium nobile Lindl Alkaloids on Posterior Capsular Opacification in Rats

作　　者：时应兰 李军 石谍 陈静 黄朝霞 SHI Ying-lan;LI Jun;SHI Die;CHEN Jing;HUANG Zhao-xia(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025

出　　处：《精细化工中间体》2024年第6期20-24,共5页Fine Chemical Intermediates

基　　金：贵州省科技计划项目(黔科合基础-ZK[2021]一般427);贵中医博士启动基金[2020]58号。

摘　　要：探讨了金钗石斛总生物碱(DNLA)对SD大鼠后发性白内障的影响。取40只健康SD大鼠随机分为空白组、模型组和3个模型组+DNLA(高/中/低)处理组。建模后连续灌胃给药30d。采用裂隙灯生物显微镜检查大鼠晶状体混浊度,HE染色观察大鼠晶状体组织病理变化,免疫荧光检测大鼠晶状体上皮细胞中α-平滑肌肌动蛋白(α-SMA)的表达。结果表明,在裂隙灯下晶状体后囊膜混浊程度为模型组>低剂量组>中剂量组>高剂量组。给药后SD大鼠眼球晶状体组织晶状体上皮增生、炎性细胞浸润和纤维组织增生等病变有明显缓减,晶状体上皮细胞中α-SMA蛋白表达量明显减弱。金钗石斛总生物碱对大鼠后发性白内障晶状体上皮细胞的异常增生及纤维化具有明显抑制效果。To investigate the effects of Dendrobium nobile Lindl alkaloids(DNLA)on posterior capsular opacification(PCO)in SD rats.Forty healthy SD rats were randomly divided into five groups:Blank group,Model group,and 3 Model group+DNLA(high/medium/low dose)treatment groups.After modeling,the drugs were administered by gavage for 30 consecutive days.Slit-lamp biomicroscopy was used to examine the turbidity of rat lens,hematoxylin and eosin(HE)staining was used to observe the histopathological changes of rat lens,and immunofluorescence was used to detect the expression ofα-smooth muscle actin(α-SMA)in the epithelial cells of rat lens.The turbidity of the posterior capsule membrane of the lens observed under the slit lamp was in the order:model group>low dose group>medium dose group>high dose group.The lesions such as lens epithelial hyperplasia,inflammatory cell infiltration and fibrous tissue hyperplasia in the lens tissue of SD rats were significantly slowed down after administration of the drug,and the expression ofα-SMA protein in the lens epithelial cells was significantly attenuated.DNLA used on PCO rats caninhibit the abnormal proliferation and fibrosis of lens epithelial cells.

关 键 词：金钗石斛总生物碱 后发性白内障 晶状体上皮细胞损伤 Α-平滑肌肌动蛋白 晶状体膜囊损伤 

分 类 号：TQ283[化学工程—有机化工]