浅谈荧光检测分析仪器中的光学滤光片  

Discussion on optical filters in fluorescence detection and analysis instruments

作　　者：高鹏[1] 王忠连[1] 班超 董明 任少鹏[1] 王瑞生[1] GAO Peng;WANG Zhonglian;BAN Chao;DONG Ming;REN Shaopeng;WANG Ruisheng(Shenyang Academy of Instrumentation Science Co.,Ltd.,Shenyang 110043,China)

机构地区：[1]沈阳仪表科学研究院有限公司,辽宁沈阳110043

出　　处：《光学仪器》2024年第6期88-94,共7页Optical Instruments

摘　　要：滤光片广泛应用于各种光学仪器中,其性能直接决定着仪器的精度和可靠性,是仪器的核心光学元件。以应用于荧光检测分析仪器中的滤光片为着眼点,论述了滤光片在仪器中的工作原理和工作过程,剖析了滤光片在光谱设计中应遵循的设计理念;介绍了设计滤光片所应用的光学薄膜技术以及主要的镀膜设备类型,通过对比分析不同设备的优缺点,对镀膜设备的选择给出了建议;根据荧光检测分析仪器的发展趋势,归纳了滤光片逐渐呈现出的发展新要求:单片集成化、光谱复杂化、低波前畸变和更深更锐截止。Filters are core optical elements in various optical instruments.The performance of these filters directly determines the accuracy and reliability of these instruments.Focusing on filters used in fluorescence detection and analysis instruments,their working principle was discussed,and the principles that should be followed when designing these filters were analyzed;the optical coating technology used for filter design and the types of main coating equipment were introduced.Suggestions were given for the selection of coating equipment by comparing and analyzing the advantages and disadvantages of different types of coating equipment.Based on the development and changes of fluorescence detection and analysis instruments,new requirements for these filters were summarized,such as coating on one substrate,more complex spectra,lower wave front distortion,deeper blocking and sharper steepness.

关 键 词：荧光 光学滤光片 检测分析仪器 光学薄膜 

分 类 号：O436[机械工程—光学工程] TB43[理学—光学]