甜瓜蔓枯病生物学特性及防治药剂筛选  

Biological Characteristics of Melon Vine Blight and Screening of Preventive Agents

作　　者：李洋[1] 赵飞 邢鲲 邓洁玉 樊思莹 蒲秀兰 杨存第 LI Yang;ZHAO Fei;XING Kun;DENG Jieyu;FAN Siying;PU Xiulan;YANG Cundi(College of Plant Protection,Shanxi Agricultural University,Taiyuan 030031,China;Departmentof Biology and Food Engineering,Lyuliang University,Lvliang 033000,China;Fanzhi CountyAgricultural Industry Development Center,Xinzhou 034000,China)

机构地区：[1]山西农业大学植物保护学院,山西太原030031 [2]吕梁学院生物与食品工程系,山西吕梁033000 [3]繁峙县农业产业发展中心,山西忻州034000

出　　处：《中国果菜》2024年第12期84-89,共6页China Fruit & Vegetable

基　　金：山西农业大学杂粮研究院科研项目(ZL20230501)。

摘　　要：为明确甜瓜蔓枯病病原菌的生物学特性以及筛选出适合防治该病症的药剂,本试验在不同温度、pH、碳源和氮源条件下对该病原菌进行培养,确定对该病原菌生长最适温度、pH、碳源、氮源。采用菌丝生长速率法,用3种杀菌剂与PDA培养基混合制成含药培养基,测定抑制率,构建毒力回归方程并计算EC_(50)。结果表明,甜瓜蔓枯病病原菌最适温度为25℃,最适pH为6,最适碳源为葡萄糖,最适氮源为蛋白胨和硝酸钾。在供试的3种药剂中,百菌清对其抑制效果最好,EC_(50)为2.097;其次为三唑酮,EC_(50)为2.494;代森锰锌的作用效果最差,EC_(50)为2.531。探讨不同环境条件对甜瓜蔓枯菌生长的影响,能够助力环保有效生物防治药剂的筛选,为甜瓜产业的可持续发展提供科学依据和技术支持。In order to clarify the biological characteristics of the pathogen of melon cran blight and select the drugs suitable for the prevention and treatment of the disease,this test cultured the pathogen under different temperature,pH,carbon source and nitrogen source conditions,and determined the most favorable temperature,pH,carbon source and nitrogen source for the growth of the pathogen.Using the mycelium growth rate method,three fungicides and PDA culture medium were mixed to make a drug-containing culture medium to determine the inhibition rate and build a virulence regression to calculate the 50.The results showed that the most suitable temperature of melon blight pathogens was 25℃,the most suitable pH was 6,the most suitable carbon source was glucose,and the most suitable nitrogen source was protein and potassium nitrate.Among the three agents tested,Bamoxazole had the best inhibition effect,with 50 of 2.097,followed by Triazolone,with 50 of 2.494;and the worst effect of Daisen manganese zinc,with 50 of 2.531.Exploring the effects of different environmental conditions on the growth of could effectively assist in the screening of environmentally friendly and effective biological control agents,providing scientific basis and technical support for the sustainable development of the melon industry.

关 键 词：甜瓜蔓枯病 产孢量 生物学特性 药剂筛选 

分 类 号：Q93-335[生物学—微生物学]