Identification of Active Components in Humic Acids Affecting Tomato Germination  

作　　者：Wei Shiping Shi Peihua Wang Quanzhi Wang Yuanhua Zhao Jiayin Yin Kaijun Wu Meng 

机构地区：[1]College of Agronomy and Horticulture,Jiangsu Vocational College of Agriculture and Forestry,Zhenjiang 212400,Jiangsu,China [2]Jiangsu Engineering and Technology Center for Modern Horticulture,Zhenjiang 212400,Jiangsu,China [3]State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 211135,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2024年第4期1-15,共15页东北农业大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(42207371);the Technological Project of Jiangsu Vocational College of Agriculture and Forestry(2021kj17);Yafu Technology Innovation and Service Major Project of Jiangsu Vocational College of Agriculture and Forestry(2024kj01);Key Research Projects of Jiangsu Vocational College of Agriculture and Forestry(2023kj14)。

摘　　要：Humic acids can promote the germination of many vegetable seeds,but the key active components remain unclear.This study utilized nutrient content,cross polarization magic angle spin ^(13)C solid magnetic resonance(CPMAS-^(13)C-NMR)and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)to characterize the chemical components of humic acids.Tomato seed germination index(GI)was determined with the goal of screening the key active components of humic acids.Humic acids had a significantly higher nutrient content,except for the total nitrogen(TN)and the total phosphorus(TP)contents.Humic acids had a higher content of O-CH_(3)/NCH,aromatic C-O and carbonyl C compared to weathered coal,with significantly lower anomeric C,aromatic C and O-alkyl C/alkyl C.There were 611 different compounds identified among the test materials using UHPLC-MS.Humic acids also had a significantly higher GI(158.0%and 153.1%)than weathered coal(85.5%).The organic matter(OM),TP and available potassium(AK)contents in humic acids were significantly positively correlated with GI,and available phosphorus(AP)was significantly negatively correlated.Among the carbon components,O-CH3/NCH,aromatic C-O and O-alkyl C/alkyl C were significantly positively correlated with GI,while anomeric C was significantly negatively correlated.Furthermore,among the top 10 positive and five negative correlation compounds,lipids and lipid-like molecules[armexifolin,boviquinone 4,3-methyladipic acid,lxocarpalactone A,monic acid,DG(20:1(11Z)/18:4(6Z,9Z,12Z,15Z)/0:0),and brassinolide]and organic acids and derivatives(N-acetylglutamic acid,8-hydroxy-5,6-octadienoic acid,acetyl-L-tyrosine,and hydroxyprolyl-methionine)in humic acids might be crucial active components for improving tomato seed germination.The results provided direct evidence for the identification of bioactive molecules of humic acids,and a scientific basis for the precise utilization of bioactive molecular components of humic acids in sustainable agricultural development.

关 键 词：humic acid cross polarization magic angle spin 13C solid magnetic resonance(CPMAS-13C-NMR) ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS) germination index(GI) active component 

分 类 号：S606[农业科学—园艺学]