Construction of Plant Expression Vector for Avian Influenza Virus HA Gene and Its Expression in Tobacco  

作　　者：Wang Yudan Wang Tingting Wang Meiliang Li Xinzhi Zhang Yao Chen Xiuling Qiu Youwen Wang Aoxue 

机构地区：[1]College of Life Sciences,Northeast Agricultural University,Harbin 150030,China [2]College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2024年第4期66-73,共8页东北农业大学学报（英文版）

基　　金：Supported by the Natural Science Foundation of Heilongjiang Province(LH2021C032)。

摘　　要：Avian influenza virus is one of the main pathogens causing avian influenza.In this experiment,the avian influenza hemagglutinin(HA)gene was transferred into tobacco by Agrobacterium tumefaciens-mediated method using kanamycin as a resistance marker to produce HA type edible vaccine designed for avian influenza hemagglutinin protein.The fusion of cholera toxin B subgene(CTB)and avian influenza HA was initiated by CaMV35S promoter,and then transformed into Nicotiana benthamiana to induce callus and adventitious bud differentiation,bud elongation,and growth and root induction.The total genomic DNA of 28 transgenic tobacco plants was extracted,and the CTB sequence of the CTB-HA fusion gene was used as a template to design primers for PCR amplification.Eight of them were positive,and four of them were expressed at the RNA level after RNA extraction and RT-PCR amplification.In western blot detection of protein extraction,two strains were shown at the position of the target protein.The results provided material support for the preparation of a transgenic plant oral vaccine against HA infection,and provided a theoretical basis for accelerating the development of a transgenic plant vaccine.

关 键 词：avian influenza CTB-HA TOBACCO transgenic plant vaccine 

分 类 号：Q819[生物学—生物工程]