施用生物菌肥对地黄重茬土壤的影响  

Effect of applying bacterial manure on the continuous cropping soil of Rehmannia glutinosa

作　　者：余彦鸽 代丹丹[1] 腊贵晓[1] 理向阳[1] 郭晓阳[1] 王艳红 杨铁钢[1] YU Yan-ge;DAI Dan-dan;LA Gui-xiao;LI Xiang-yang;GUO Xiao-yang;WANG Yan-hong;YANG Tie-gang(Institute of Traditional Chinese Medicine,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院中药材研究所,郑州450002

出　　处：《湖北农业科学》2024年第12期99-104,共6页Hubei Agricultural Sciences

基　　金：国家现代农业产业技术体系资助项目(CARS-21);河南省中药材体系项目(HARS-22-11-G2);河南省农业科学院新兴学科发展专项(2024XK04)。

摘　　要：以地黄(Rehmannia glutinosa)的重茬土壤为研究对象,分析生物菌肥对地黄重茬土壤的微生物群落构成及土壤内根系残留化合物的影响。通过施用不同浓度的生物菌肥,结合土壤微生物计数法和超高效液相色谱-四极杆飞行时间质谱法(UPLC-QTOF/MS)对施有生物菌肥的重茬土壤分别进行微生物种群计数和根系残留化合物分析。结果表明,不同处理下土壤微生物群落结构存在明显差异,其中,0.05%生物菌肥施用60 d后的地黄重茬土壤中细菌、放线菌之和最高,而真菌数量相对较少。筛选出9种地黄重茬土壤中特有的根系残留化合物,其中,在生物菌肥浓度为0.05%时,化合物2的含量最低;化合物5和化合物6含量在生物菌肥浓度为0.10%、施用60 d的条件下最低。可见,外施生物菌肥可有效改变地黄重茬土壤微生物数量和降解重茬土壤中根系残留的某些化合物,有效缓解地黄的连作障碍。Taking the continuous cropping soil of Rehmannia glutinosa as the research object,the effect of applying bacterial manure on microbial community composition and root residual compounds in the soil of Rehmannia glutinosa was analyzed.The soil microbial count method was used to detect the number of microorganisms.Ultra high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS)was used to profile the root residual compounds in the soil of Rehmannia glutino⁃sa.The results showed that soil microbial community structure had significant differences under different treatments.The total number of bacteria and actinomycetes in Rehmannia glutinosa soil after 60 d of applying 0.05%bacterial manure was the highest,while the number of fungi was less.Nine unique residue compounds were screened out in the soil of Rehmannia glutinosa.The content of Com⁃pound 2 was the lowest when the concentration of bacterial manure was 0.05%.At the same time,the contents of Compound 5 and Compound 6 were the lowest when the concentration of bacterial manure was 0.10%and applied for 60 days.It was found that the appli⁃cation of bacterial manure could effectively change the soil microbial populations and reduce some residual compounds of continuous cropping soil.It was also beneficial to alleviate the continuous cropping obstacle of Rehmannia glutinosa.

关 键 词：生物菌肥 地黄(Rehmannia glutinosa) 重茬土壤 UPLC-QTOF/MS 

分 类 号：S567.234[农业科学—中草药栽培] S154[农业科学—作物学]