洪山菜薹抗根肿病新品系的创制  

Development of new Hongshancaitai line with clubroot resistance

作　　者：任志勇 董斌峰 何青松 聂启军[1] REN Zhi-yong;DONG Bin-feng;HE Qing-song;NIE Qi-jun(Institute of Economic Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院经济作物研究所,武汉430064

出　　处：《湖北农业科学》2024年第12期121-124,198,共5页Hubei Agricultural Sciences

基　　金：湖北省自然科学基金青年项目(2023AFB246);国家大宗蔬菜产业技术体系项目(CARS-23-G28);湖北省支持种业高质量发展资金项目(HBZY2023B004)。

摘　　要：以抗根肿病红菜薹(Brassica rapa ssp.chinensis var.purpurea)种质资源GD1为抗源供体,以优异洪山菜薹早熟品系DGZ1902为背景材料进行回交转育,针对抗源携带的抗病基因CRzi8开发PARMS型分子标记,进一步结合分子标记辅助选择、单株优选以及根肿病接种鉴定,创制抗根肿病洪山菜薹新品系。结果表明,开发的标记可准确鉴定回交世代群体的抗性单株。田间病圃抗性鉴定及农艺性状观察表明,创制的洪山菜薹抗根肿病品系CR-DGZ1902高抗根肿菌4号生理小种,同时具有洪山菜薹的农艺性状特征及DGZ1902的早熟特性。Using clubroot resistance(CR)Brassica rapa ssp.chinensis var.purpurea germplasm GD1 as the resistant donor,and using DGZ1902,an early mature strain of Hongshancaitai,as the background material,backcross breeding was conducted.The penta-prim⁃er amplification refractory mutation system(PARMS)marker for CR gene CRzi8 carried by CR donor was developed and furtherly ap⁃plied in the marker assistance selection(MAS),single plant selection and identification of root disease inoculation,finally the new CR Hongshancaitai line was created.The results showed that the PAMRS marker for CRzi8 could accurately detect the CR individuals from the backcross breeding program.Field resistance test and agronomic traits evaluation indicated that the developed CR Hongshan⁃caitai line CR-DGZ1902 was resistant to Plasmodiophora brassicae pathotype 4,and had both agronomic characteristics of Hongshan⁃caitai and early maturity characteristics of DGZ1902.

关 键 词：洪山菜薹 分子标记 根肿病 抗病基因 新品系 

分 类 号：S634.6[农业科学—蔬菜学]