灵芝响应木质素转录因子基因鉴定及分析  

Identification and Analysis of Lignin-Responsive Transcription Factors in Ganoderma lucidum

作　　者：王丽宁 王庆福 WANG Lining;WANG Qingfu(Institute of Biological and Medical Engineering,Guangdong Academy of Sciences,Guangdong Engineering Research&Development Center for Comprehensive Utilization of Plant Fiber,Guangdong Engineering Laboratory of Biomass Value-added Utilization,Guangzhou Key Laboratory for Comprehensive Utilization of Plant Fiber,Guangzhou 510316,Guangdong,China)

机构地区：[1]广东省科学院生物与医学工程研究所,广东省植物纤维综合利用工程技术研究开发中心,广东省生物质高值化利用工程实验室,广州市植物纤维综合利用重点实验室,广东广州510316

出　　处：《食用菌学报》2024年第6期17-28,共12页Acta Edulis Fungi

基　　金：国家自然科学基金(32202570)。

摘　　要：基于灵芝(Ganoderma lucidum)菌株GL0102以葡萄糖和木质素为碳源培养菌丝的转录组数据,鉴定差异表达转录因子基因并验证,研究差异表达转录因子基因在以6种农林废弃物(甘蔗渣,稻草,麦秆,栎树、杉树、松树木屑)为培养基碳源条件下的表达情况。构建差异表达基因chr8g0158731的干扰转化子,测定3个转化子在不同碳源条件下菌丝生长速度和对木质素的利用情况。结果表明:与以葡萄糖为碳源培养的菌丝相比,以木质素为碳源培养的菌丝转录组中分别有26个上调表达、40个下调表达的转录因子基因。在以木质素为碳源培养的菌丝中表达上调的7个转录因子基因,在分别以6种农林废弃物为碳源培养的菌丝中表达量较高;在以木质素为碳源培养的菌丝中表达下调的6个转录因子基因,在分别以6种农林废弃物为碳源培养的菌丝中表达量较低。在以葡萄糖为碳源培养时,3个转化子菌丝生长速度与野生型菌株的无明显差异;在分别以木质素和6种农林废弃物为碳源培养时,与野生型菌株比较,3个转化子的菌丝生长速度均增加,chr8g0158731的表达量均降低。以木质素为碳源培养时,3个转化子对木质素的降解程度均高于野生型菌株。研究结果可为后续研究木质素降解提供候选基因。Lignin-responsive transcription factors in Ganoderma lucidum were identified through comparison of the transcriptome data of G.lucidum GL0102 mycelia cultured with glucose as the carbon source versus lignin as the carbon source.Differentially expressed transcription factors(TFs)were identified,validated,and then determined for expression levels under different agricultural and forestry wastes(bagasse,rice straw,wheat straw,oak sawdust,spruce sawdust,and pine sawdust).Three interference strains of the differential transcription factor chr8g0158731 were constructed,and then determined for mycelial growth rate under different carbon sources and lignin utilization.The results showed that there were 26 up-regulated and 40 down-regulated TFs in the transcriptome of mycelia cultured on lignin compared with that of mycelia cultured on glucose.When cultured on the agricultural and forestry wastes,seven of the up-regulated TFs showed a high expression level in mycelia,and six of the down-regulated TFs showed a low expression level.When cultured with glucose as the carbon source,the mycelial growth rates of the three interference strains were not significantly different from that of the wild-type strain.When cultured on lignin and the six agricultural and forestry wastes,the mycelial growth rates of the three interference strains were greater than that of the wild-type strain,and the expression level of chr8g0158731 in the the three interference strains was lower than that in the wild-type strain.When cultured with lignin as the carbon source,all three interference strains degraded lignin to a greater extent than the wild-type strain.The results provided candidate genes for subsequent lignin degradation studies.

关 键 词：灵芝 转录因子 木质素 转录组 

分 类 号：S567.31[农业科学—中草药栽培]