桦褐孔菌乙酸乙酯萃取物对高尿酸血症模型小鼠的降尿酸作用  

Ethyl Acetate Extract of Inonotus obliquus Reduced Uric Acid in Hyperuricemia Model Mice

作　　者：林宇琪 马吉胜 包海鹰[1] LIN Yuqi;MA Jisheng;BAO Haiying(College of Chinese Materia Medica,Jilin Agricultural University,Changchun 130118,Jilin,China;School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou 325000,Zhejiang,China)

机构地区：[1]吉林农业大学中药材学院菌类中药资源开发与利用重点研究室,吉林长春130118 [2]温州医科大学药学院,浙江温州325000

出　　处：《食用菌学报》2024年第6期81-91,共11页Acta Edulis Fungi

基　　金：长江学者与创新团队项目(IRT-15R25)。

摘　　要：为探究桦褐孔菌(Inonotus obliquus)乙酸乙酯萃取物的降尿酸作用,将小鼠随机分为空白组、模型组、阳性BA组(别嘌呤醇5 mg·kg^(-1))、阳性BB组(苯溴马隆7.8 mg·kg^(-1))和桦褐孔菌乙酸乙酯萃取物低剂量组(50 mg·kg^(-1))、高剂量组(100mg·kg^(-1)),除空白组外,其余各组每天腹腔注射氧嗪酸钾(300 mg·kg^(-1))联合灌胃次黄嘌呤(500 mg·kg^(-1))建立高尿酸血症模型。造模成功后按照分组进行相应的处理,每天1次,连续28 d,末次给药1 h后,采用酶比色法测定小鼠血液的尿酸、尿素氮、肌酐含量和黄嘌呤氧化酶、腺苷脱氨酶活性,计算肾脏、肝脏指数,苏木素-伊红染色法观察肾脏、肝脏病理变化,酶联免疫吸附测定法检测小鼠肾脏中URAT1、GLUT9蛋白含量,免疫组织化学染色观察肾脏组织中URAT1、GLUT9蛋白表达位置,蛋白免疫印迹法检测URAT1、GLUT9蛋白表达水平。结果表明:与模型组相比,各给药组小鼠肾脏、肝脏指数降低,尿酸、尿素氮、肌酐含量及黄嘌呤氧化酶、腺苷脱氨酶活性降低,肾脏、肝脏组织病理状况明显改善,肾脏中URAT1、GLUT9含量减少,肝脏中XOD活性降低,肾脏组织中URAT1、GLUT9蛋白定位表达,AOD值降低,URAT1、GLUT9蛋白表达量下调。研究结果表明,桦褐孔菌乙酸乙酯萃取物具有降尿酸作用,且可有效改善高尿酸血症所致的肾脏及肝脏损伤。The uric acid lowering effect of ethyl acetate extract from Inonotus obliquus on hyperuricemic mice was studied.Mice were randomly divided into the following groups:normal group,hyperuricemia model group,allopurinol group(5 mg·kg^(-1),positive BA),benzbromarone group(7.8 mg·kg^(-1),positive BB),low-dose I.obliquus ethyl acetate extract(IO-E)group at 50 mg·kg^(-1),and high-dose I.obliquus ethyl acetate extract group at 100 mg·kg^(-1).Except the normal group,all the other groups of mice were administered potassium oxyzinate(300 mg·kg^(-1))intraperitoneally and hypoxanthine(500 mg·kg^(-1))by gavage for 14 consecutive days to establish a hyperuricemia model.After successful modeling,mice in different groups were administered corresponding interventions once a day for 28 consecutive days.One hour after the last administration,mice in different groups were measured for levels of uric acid,urea nitrogen,creatinine,xanthine oxidase,and adenosine deaminase in the blood by enzyme colorimetry,calculated for kidney and liver indices,observed for pathological changes in the kidneys and livers by hematoxylin-eosin staining,determined for URAT1 and GLUT9 protein contents in the kidneys by enzyme linked immunosorbent assay,observed for the localization of URAT1 and GLUT9 in the kidney tissues by immunohistochemical staining,and determined for the expression of URAT1 and GLUT9 by immunoblotting.Compared with the model group,mice in positive BA,positive BB and I.obliquus ethyl acetate extract groups showed ameliorated hyperuricemic symptoms,including decreased kidney and liver indices,decreased levels of blood uric acid,urea nitrogen and creatinine,decreased xanthine oxidase and adenosine deaminase activity,and ameliorated pathological changes in the kidney and liver tissues.The levels of URAT1 and GLUT9 in the kidneys of BA,BB and IO-E treated mice were significantly reduced,and the xanthine oxidase activity in the livers of these groups was also significantly reduced.Immunohistochemical staining of URAT1 and GLUT showed th

关 键 词：高尿酸血症 桦褐孔菌 乙酸乙酯 尿酸转运蛋白 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]