基于非靶向代谢组学分析两种混合青稞麸皮差异代谢物  

Analysis of Differential Metabolites in Two Types of Mixed Barley Bran Based on Non-Targeted Metabolomics

作　　者：奉综涛 买地那·帕尔哈提 次仁普赤 禄亚洲 黄明贵 FENG Zongtao;MAIDINA Paerhati;CIREN Puchi;LU Yazhou;HUANG Minggui(Food Science College,Xizang Agriculture and Animal Husbandry University,Linzhi,Xizang,860000;Xizang Qizheng Tibetan Medicine Incorporated Company,Lasa,Xizang,850000,China)

机构地区：[1]西藏农牧学院食品科学学院,西藏林芝860000 [2]西藏奇正藏药股份有限公司,西藏拉萨850000

出　　处：《高原农业》2025年第1期57-64,共8页Journal of Plateau Agriculture

基　　金：南京农业大学—西藏农牧学院联合项目(202106);西藏农牧学院研究生教育创新计划项目(YJS2023-06);西藏农牧学院学科建设项目(533323001)。

摘　　要：本研究旨在通过非靶向代谢组学分析两种混合青稞麸皮的差异代谢物。采用非靶向代谢组学研究方法,通过主成分分析(principal component analysis,PCA)和聚类分析等统计学方法对两种混合青稞麸皮进行差异分析。结果表明:两种混合青稞麸皮共检测出21类共6435种代谢物,共筛选出549种差异显著代谢物,占总代谢物的8.53%,其中上调280个,下调269个;通过对差异代谢物富集的代谢途径分析,发现两种混合青稞麸皮中的差异代谢物主要存在于亚油酸途径,甘氨酸、丝氨酸和苏氨酸代谢途径,抗坏血酸和阿糖二酸代谢途径,甘油磷脂代谢途径及类黄酮生物合成途径。该研究确定了两种混合青稞麸皮的差异代谢物,为进一步研究其营养成分和功能活性成分提供重要参考。The aim of this study is to analyze the differential metabolites of two mixed barley bran using non-targeted metabolomics.Using non-targeted metabolomics research methods,statistical methods such as principal component analysis(PCA)and cluster analysis were used to analyze the differences between two types of mixed barley bran.The results showed that 21 categories of 6435 metabolites were detected in the two mixed barley bran samples,and a total of 549 significantly different metabolites were screened out,accounting for 8.53%of the total metabolites.Among them,280 were upregulated and 269 were downregulated;Through the analysis of metabolic pathways enriched with differential metabolites,it was found that the differential metabolites in the two mixed barley bran mainly existed in the linoleic acid pathway,glycine,serine,and threonine metabolism pathways,ascorbic acid and arabinose metabolism pathways,glycerophospholipid metabolism pathway,and flavonoid biosynthesis pathway.This study identified the differential metabolites of two mixed barley bran,providing important references for further research on their nutritional and functional active ingredients.

关 键 词：青稞麸皮 非靶向代谢组学 差异代谢物 

分 类 号：Q591.1[生物学—生物化学]