基于荧光光谱和拉曼光谱技术的中药检测研究  

Fluorescence Spectroscopy and Raman Spectroscopy Techniques

作　　者：逯美红[1] 张凡 暴雅婷 王志军[1] 雷海英[3] 王翔宇 高鹏慧 LU Mei-hong;ZHANG Fan;BAO Ya-ting;WANG Zhi-jun;LEI Hai-ying;WANG Xiang-yu;GAO Peng-hui(Department of Physics,Changzhi University,Changzhi 046011,China;Physics Experiment Center,Xi'an Information Vocational University,Xi'an 710125,China;Department of Life Sciences,Changzhi University,Changzhi 046011,China)

机构地区：[1]长治学院物理系,山西长治046011 [2]西安信息职业大学物理实验中心,陕西西安710125 [3]长治学院生命科学系,山西长治046011

出　　处：《光谱学与光谱分析》2025年第1期139-145,共7页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(11905018);山西省高等学校教学改革创新项目(J20221074);山西省高等院校科技开发项目(2021L517)资助。

摘　　要：中医药是中华文化的瑰宝和中华文明的结晶,在全民健康中发挥着重要作用。由于中药生长环境受限及市场需求量的增大,市场上出现了许多以次充好、以假乱真的中药,这对中药发展及质量保障都极为不利。因此,对中药的检测和鉴定非常重要。为了探究中药的荧光光谱和拉曼光谱特性,选取白芷、苏木、山药、地榆四种中药进行分类检测研究。一方面,从化学成分和结构的角度分析中药白芷和苏木产生荧光的可能性,采用F-4600型荧光光谱仪(200~750 nm)分别在不同激发波长和不同浓度下测试了白芷和苏木水浸液的荧光光谱,探究了白芷和苏木水浸液的荧光光谱特性,并对荧光强度与不同浓度、激发波长的关系进行了探讨。另一方面,采用共聚焦显微拉曼光谱仪(100~4000 cm^(-1))对4种不同产地的山药和2种不同产地的地榆中药饮片的拉曼光谱分别进行测试研究。结果表明,白芷水浸液在波长260~350 nm的光激发下,产生较强荧光,最佳激发波长是340 nm,荧光峰值波长为420 nm,其荧光强度随浓度的增大而增强,且样品浓度较低时,荧光强度与浓度成线性关系;苏木水浸液在200~290 nm的光激发下,产生较强荧光,最佳激发波长为220 nm,荧光峰值波长为345 nm,随浓度的增加,荧光强度先增强后减弱,在浓度为0.175 mg·mL^(-1)时荧光强度达到最大。白芷水浸液和苏木水浸液的荧光强度都与激发波长的关系呈现高斯分布,符合荧光规律。分析山药和地榆的拉曼光谱,发现不同产地的拉曼特征基本一致,确认山药的拉曼特征峰主要集中在477、862、939、1080、1258、1337和1457 cm^(-1)处;地榆的拉曼特征峰主要集中在862和1337 cm^(-1)处,主要归属与已有的化学成分研究结果相符。部分特征峰处拉曼活性出现差异,可以作为区分不同产地中药的依据。该研究结果不仅为荧光光谱在中药鉴定及质量分析中的应用�Traditional Chinese medicine is a treasure of Chinese culture and the crystallization of Chinese civilization,playing an important role in the health of the whole nation.Due to the limited growth environment of traditional Chinese medicine and the increasing market demand,numerous substandard and falsified traditional Chinese medicines have emerged in the market,which is extremely detrimental to the development and quality assurance of traditional Chinese medicine.Therefore,it is of utmost importance to detect and identify traditional Chinese medicine.Four medicinal herbs,angelica dahurica,Sappanwood,yam,and sanguinaria officinalis,were selected for classification and detection research to explore traditional Chinese medicine's fluorescent and Raman spectral characteristics.On the one hand,in this paper,we analyzed the possibility of angelica dahurica and sappanwood,traditional Chinese medicinal herbs,to exhibit fluorescence from the perspectives of chemical composition and structure.An F-4600 fluorescence spectrophotometer(200~750 nm)was used to measure the fluorescence spectra of aqueous extracts of angelica dahurica and sappanwood at different excitation wavelengths and concentrations,investigating the fluorescence spectral characteristics of aqueous extracts of angelica dahurica and sappanwood,and discussing the relationship between fluorescence intensity and different concentrations and excitation wavelengths.On the other hand,a confocal micro-Raman spectrometer(100~4000 cm^(-1))was used to conduct Raman spectroscopy tests on herbal slices of yam from four different regions and sanguisorba officinalis from two different regions,and then the Raman spectra were obtained.The results showed that the aqueous extract of angelica dahurica exhibited strong fluorescence under excitation wavelengths ranging from 260 to 350 nm.The optimal excitation wavelength is 340 nm,and the peak fluorescence wavelength is 420nm.The fluorescence intensity increased with the concentration increase,following a linear relationship at l

关 键 词：白芷 苏木 山药 地榆 荧光光谱 拉曼光谱 

分 类 号：O657.37[理学—分析化学]