MEBT/MEBO通过调控TXNIP/NLRP3/Caspase-1信号通路促进糖尿病创面愈合  

MEBT/MEBO promotes diabetic wound healing by regulating TXNIP/NLRP3/Caspase⁃1 pathway

作　　者：姚明哲 竺仕林 李文武 杨雅量 葛星月 唐乾利[2,3] YAO Mingzhe;ZHU Shilin;LI Wenwu;YANG Yaliang;GE Xingyue;TANG Qianli(Graduate School,Youjiang Medical University for Nationalities,Baise 533000,China;Life Science and Clinical Medicine Research Center,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,China;Guangxi University of Chinese Medicine/Guangxi Scientific Research Center of Traditional Chinese Medicine,Nanning 530200,China)

机构地区：[1]右江民族医学院研究生院,广西百色533000 [2]右江民族医学院附属医院生命科学与临床医学研究中心,广西百色533000 [3]广西中医药大学/广西中医药科学实验中心,广西南宁530200

出　　处：《海南医学院学报》2024年第24期1848-1858,共11页Journal of Hainan Medical University

基　　金：国家自然科学基金面上项目(81774327);广西特聘专家项目[桂人才通字(2019)13号];广西医学高层次骨干人才“139”计划培养人选培养专项资助[桂卫科教发(2018)22号];右江民族医学院研究生创新计划项目(YXCXJH2023003)。

摘　　要：目的:探讨湿润暴露疗法/湿润烧伤膏(moist exposed burn therapy/moist exposed burn ointment,MEBT/MEBO)对糖尿病创面中TXNIP/NLRP3/Caspase-1信号通路表达的影响。方法:将48只Wistar雄性大鼠随机平均分为对照组、模型组、美宝组和贝复新组。其中对照组构建急性创面模型,其余组构建糖尿病创面模型。随后美宝组和贝复新组分别采用湿润烧伤膏和重组牛碱性成纤维生长因子进行换药,对照组及模型组采取生理盐水进行换药。通过H&E染色、免疫荧光法及实时荧光定量PCR检测观察各组创面第3、7、14天愈合情况及TXNIP、NLRP3及Caspase-1表达情况。结果:(1)治疗第3天,对照组创面愈合率高于模型组,且具有统计学差异性(P<0.05);治疗第7、14天,对照组、美宝组和贝复新组创面愈合率高于模型组(P<0.05)。(2)治疗第3、7、14天,对照组、美宝组和贝复新组的TXNIP、NLRP3表达量低于模型组(P<0.05):治疗第3、7、14天,对照组和美宝组的Caspase-1表达量低于模型组(P<0.05)。结论:MEBT/MEBO可通过抑制TXNIP/NLRP3/Caspase-1通路表达,减轻糖尿病创面的炎症反应,从而促进糖尿病创面的愈合。Objective:To investigate the effect of moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)on the expression of TXNIP/NLRP3/Caspase-1 pathway in diabetic wounds.Methods:A total of 48 male Wistar rats were randomly divided into the control group,the model group,the MEBO group and the rb-bFGF group.The acute wound model was established in the control group,and the diabetic wound model was established in the other groups.Then the MEBO group and the rb-bFGF group were treated with moist burn ointment and recombinant bovine basic fibroblast growth factor,respectively.The control group and the model group were treated with normal saline.The wound healing and the expression of TXNIP,NLRP3,and Caspase-1 in each group were observed by H&E staining,immunofluorescence method,and real-time qPCR on the 3rd,7th,and 14th day.Results:⑴On the 3rd day of treatment,the wound healing rate of the control group was higher than that of the model group,and the difference was statistically significant(P<0.05).On the 7th and 14th day of treatment,the wound healing rates of the control group,MEBO group,and rb-bFGF group were higher than those of the model group(P<0.05).⑵On the 3rd,7th,and 14th day of treatment,the expression levels of TXNIP and NLRP3 in the control group,MEBO group,and rb-bFGF group were lower than those in the model group(P<0.05).On the 3rd,7th and 14th day of treatment,the expression levels of Caspase-1 in the control group and MEBO group were lower than those in the model group(P<0.05).Conclusion:MEBT/MEBO can inhibit the expression of TXNIP/NLRP3/Caspase-1 pathway,reduce the inflammatory response of diabetic wound,and thus promote the healing of diabetic wound.

关 键 词：湿润暴露疗法/湿润烧伤膏(MEBT/MEBO) 糖尿病创面 TXNIP NLRP3 CASPASE-1 

分 类 号：R587.1[医药卫生—内分泌]