Proteomic analysis of the biomineralization of spherical Brushite produced from a wheat bran aqueous extract  

作　　者：Iván Jalil Antón Carreño-Márquez René Renato Balandrán-Quintana José Antonio Azamar-Barrios José Ángel Huerta-Ocampo Ana María Mendoza-Wilson Gabriela Ramos-Clamont Montfort Sergio Encarnación-Guevara Magdalena Hernández-Ortiz 

机构地区：[1]Centro de Investigación en Alimentación y Desarrollo,A.C.Coordinación de Tecnología de Alimentos de Origen Vegetal,Carretera Gustavo Enrique Astiazarán Rosas,No.46,83304,Hermosillo,Sonora,Mexico [2]Centro de Investigación y de Estudios Avanzados del IPN,Unidad Mérida,Departamento de Física Aplicada,Carretera antigua a Progreso Km 6,97310,Mérida,Yucatán,Mexico [3]CONACYT-Centro de Investigación en Alimentación y Desarrollo,A.C.Coordinación de Ciencia de los Alimentos,Hermosillo,Sonora,Mexico [4]Centro de Investigación en Alimentación y Desarrollo,A.C.Coordinación de Ciencia de los Alimentos,Hermosillo,Sonora,Mexico [5]Centro de Ciencias Genómicas,UNAM,Cuernavaca,Morelos,Mexico

出　　处：《Food Bioscience》2023年第3期1951-1959,共9页食品生物科学（英文）

基　　金：supported by Consejo Nacional de Ciencia y Tecnología(CONACYT)Mexico,under project No.A1-S-40197;funded by projects FOMIX-Yucatán 2008-108160;CONACYT LAB-2009-01-123913,292692,294643,188345 and 204822.

摘　　要：Wheat bran aqueous extracts provide phosphate ions and other necessary but unknown biomolecules for synthesizing spherical microparticles of dicalcium phosphate dihydrate or Brushite,which is interesting for the food industry due to its delivering nutraceuticals properties.This study tests the hypothesis that proteins are the scaffold biomolecules over which the Brushite crystals grow.Brushite was synthesized from the wheat bran aqueous extract by adding calcium ions.Precipitated particles were characterized by x-ray diffraction,scanning electron microscopy,energy dispersive,and infrared spectroscopies,corroborating a single Brushite phase.The Brushite particles were demineralized for searching and identifying intracrystalline proteins through mass spectroscopy and bioinformatics.Six hundred and seventy-six proteins were identified in the Brushite and analyzed by their relative abundance and intrinsic disorder,giving 20 top proteins as scaffold candidates.Additionally,wheat bran aqueous extract was subjected to size exclusion chromatography to explore if a fraction is responsible for the Brushite synthesis.Four chromatographic fractions were obtained and treated as the aqueous extract to induce mineralization.Three fractions produced Brushite,from which two produced spherical brushite particles with diameters between 50 and 200μm.Particle size and morphology in one of the latter fractions matched those precipitated by the raw aqueous extract.Results strongly suggest that specific proteins in the wheat bran aqueous extract may have a role as a scaffold in spherical Brushite biomineralization.

关 键 词：Spherical brushite Wheat bran Calcium phosphates Protein scaffolds 

分 类 号：TS210[轻工技术与工程—粮食、油脂及植物蛋白工程]