基于非靶向代谢组学的丹霞梧桐不同器官代谢物分析  

Analysis of Metabolites in Different Organs of Firmiana danxiaensis Based on Untargeted Metabolomics

作　　者：莫罗坚 胡秋艳 张庆威 林艳 李子雍 黄久香 MO Luojian;HU Qiuyan;ZHANG Qingwei;LIN Yan;LI Ziyong;HUANG Jiuxiang(Dongguan Institute of Forestry Science/Forest Ecosystem Research Station in City Cluster of the Pearl River Estuary,Dongguan,Guangdong 523106,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510642,China)

机构地区：[1]东莞市林业科学研究所/广东珠江口城市群森林生态系统国家定位观测研究站,广东东莞523106 [2]华南农业大学林学与风景园林学院,广东广州510642

出　　处：《林业与环境科学》2024年第6期33-39,共7页Forestry and Environmental Science

基　　金：东莞市社会科技发展项目(2019507101550)。

摘　　要：为了探究丹霞梧桐Firmiana danxiaensis不同器官的代谢物差异及药用价值,采用气相色谱—质谱联用技术(Gas chromatography-mass spectrometry,GC-MS)对其根、茎和叶进行代谢物分析与鉴定,利用主成分分析(Principal component analysis,PCA)、正交偏最小二乘法判别分析(Orthogonal partial least squares discriminant analysis,OPLS-DA)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析对不同器官的代谢物进行筛选与富集分析。结果显示,共检测到10558个代谢物,包括5184个差异代谢物,其中根和茎的差异代谢物2965个,根和叶的差异代谢物3611个,茎和叶的差异代谢物3662个。KEGG分析结果显示,差异代谢物在黄酮和类黄酮的生物合成通路中富集较为明显,丹霞梧桐的核心差异代谢物中共有16个黄酮化合物参与代谢。To explore the differences and medicinal value of metabolites in different organs of Firmiana danxiaensis,the metabolites extracted from root,stem and leaves were analyzed and identified by Gas chromatography-mass spectrometry(GC-MS).Principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis were used to screen and enrich metabolites in different organs.The results showed that 10558 metabolites were detected,including 5184 differential metabolites.Among them,there were 2965 differential metabolites between root and stem,3611 differential metabolites between root and leaf,and 3662 differential metabolites between stem and leaf.The results of the KEGG pathway analysis showed that the differential metabolites were significantly enriched in the biosynthetic pathways of flavonoids and flavonols.A total of 16 flavonoids were found to be involved in the metabolism of the core differential metabolites of F.danxiaensis.

关 键 词：丹霞梧桐 非靶向代谢组学 差异代谢物 黄酮类 

分 类 号：S718.4[农业科学—林学]