一株萎缩芽孢杆菌XW-2对松材线虫的杀线活性  

The Activity of Bacillus atrophaeus XW-2 Against Bursaphelenchus xylophilus

作　　者：肖海燕 黄华毅 胡铭彪 龙锦发 陈刘生 黄咏槐[2] 赵丹阳[2] 扈丽丽 XIAO Haiyan;HUANG Huayi;HU Mingbiao;LONG Jinfa;CHEN Liusheng;HUANG Yonghuai;ZHAO Danyang;HU Lili(Shaoguan State Owned Forest Farm Affairs Center,Shaoguan,Guangdong 512000,China;Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China)

机构地区：[1]韶关市国有林场事务中心,广东韶关512000 [2]广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东广州510520

出　　处：《林业与环境科学》2024年第6期73-77,共5页Forestry and Environmental Science

基　　金：广东省林业科技创新项目(2023KJCX024)。

摘　　要：为明确萎缩芽孢杆菌Bacillus atrophaeus XW-2对松材线虫Bursaphelenchus xylophilus的杀线活性,开发松材线虫病生防制剂,研究通过室内浸渍试验,测定了XW-2菌悬液和发酵上清液对松材线虫的杀线活性。结果表明,XW-2的菌悬液和发酵上清液原液处理松材线虫12 h时,松材线虫死亡率分别为100%和91.00%;处理24 h的死亡率均可达100%;随着稀释倍数的增大,线虫死亡率逐渐降低,稀释100倍时,菌悬液处理松材线虫24 h的线虫死亡率为86.67%,发酵上清液处理的线虫死亡率为63.00%。因此,XW-2菌悬液和发酵上清液对松材线虫均具有杀线活性,相同浓度下的菌悬液杀线活性显著(P<0.05)高于发酵上清液。To determine the killing activity of Bacillus atrophaeus XW-2 against the Bursaphelenchus xylophilus,a biocontrol agent against pine wood nematode disease was developed.The killing activity of XW-2 bacterial suspension and fermentation supernatant against Bursaphelenchus xylophilus was determined by the indoor immersion test.The results showed that when XW-2 bacterial suspension and fermentation supernatant were used to treat pine wood nematodes for 12 hours,the correction rates for pine wood nematodes were 100%and 91.00%,respectively.However,after 24 h of treatment,the corrected mortality rates reached 100%.As the dilution concentration increases,the mortality rate of nematodes gradually decreases.However,after diluting the bacterial suspension 100 times and treating nematodes for 24 hours,the corrected mortality rate of nematodes is 86.67%.However,after diluting the fermentation supernatant 100 times and treating pine wood nema-todes for 24 hours,the corrected mortality rate of nematodes is 63.00%.Therefore,the XW-2 bacterial suspension and fermentation supernatant both had bactericidal activity against pine wood nematodes,and the bactericidal activity of the bacterial suspension at the same concentration was significantly(P<0.05)higher than that of the fermentation supernatant.

关 键 词：生防制剂 松材线虫 综合防控 

分 类 号：S763[农业科学—森林保护学]