响应面法优化降脂通脉方多糖的提取工艺研究  

Optimization of Extraction Process of Polysaccharides from Jiangzhi Tongmai Formula (降脂通脉方) by Response Surface Methodology

作　　者：刘丽桃 傅春燕 刘诗薇 周秀娟 谢雨芊 欧阳玉珍 王彪 葛金文 LIU Litao;FU Chunyan;LIU Shiwei;ZHOU Xiujuan;XIE Yuqian;OUYANG Yuzhen;WANG Biao;GE Jinwen(School of Food and Chemical Engineering,Shaoyang University,Shaoyang Hunan 422000,China;School of Pharmacy,Shaoyang University,Shaoyang Hunan 422000,China;Hunan Academy of Chinese Medicine,Changsha Hunan 410000,China)

机构地区：[1]邵阳学院食品与化学工程学院,湖南邵阳422000 [2]邵阳学院药学院,湖南邵阳422000 [3]湖南省中医药研究院,湖南长沙410000

出　　处：《中医药导报》2024年第12期58-62,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省教育厅科学研究项目(22C0460);2022年研究生科研创新项目(CX2022SY073)。

摘　　要：目的:采用Box-Behnken响应面法优选中药复方降脂通脉方(JZTM)中多糖的提取工艺。方法:通过比较浸提法、回流法、超声法、超声辅助浸提法和超声辅助回流法5种方法,得出超声辅助回流法可提高多糖得率;之后,基于单因素实验优选JZTM多糖提工艺的影响因素及其范围,运用Box-Behnk响应面设计法构建三因素三水平实验方案,得出最佳工艺条件,再进行验证。结果:超声辅助回流法的多糖提取率最高,达到12.32 mg/g;进一步优化,得出最佳工艺条件为:液料比21.7∶1(mL/g),超声时间31.19 min,回流时间2.01 h,该条件下提取率达到27.14 mg/g。结论:该提取工艺简单可行,验证实验结果可靠,为JZTM的开发利用和工业化生产提供理论参考依据。Objective:To optimize the extraction process of polysaccharide from Jiangzhi Tongmai Formula(JZTM) by Box-Behnken response surface design.Methods:By comparing extraction method,reflux method,ultrasonic method,ultrasonic-assisted extraction method and ultrasonic-assisted reflux method,it was concluded that ultrasonic-assisted reflux method could improve polysaccharide yield.After that,the influencing factors and the range of JZTM polysaccharide extraction process were optimized based on single factor experiment,and the four-factor and three-level experiment scheme was constructed by Box-Behnk response surface design method,and the optimal process conditions were obtained,and then verified.Results:The extraction rate of polysaccharide by ultrasonic-assisted reflux method was the highest,reaching 12.32 mg/g.Further optimization,the optimum process conditions were as follows:liquid-solid ratio 21.7 ∶1(m L/g),ultrasonic time 31.19 min,reflux time 2.01 h.The extraction rate under this condition was 27.14 mg/g.Conclusion:The extraction process is simple and feasible,and the experimental results are reliable.It provides a reference for the development and utilization of Jiangzhitongmai formula and industrial production.

关 键 词：降脂通脉方 多糖 超声辅助回流法 响应面法 提取工艺 

分 类 号：R284.2[医药卫生—中药学]