PEG引发对老芒麦劣变种子吸胀过程中抗氧化生理的影响  

Effect of PEG Priming on Antioxidant Physiology of Deteriorated Siberian Wildrye Seeds during Imbibition

作　　者：石金玉 王一帆[1] 毛培胜[1] SHI Jinyu;WANG Yifan;MAO Peisheng(College of Grassland Science and Technology,China Agricultural University/Beijing Key Laboratory of Pratacultural Science,Beijing 100193,China)

机构地区：[1]中国农业大学草业科学与技术学院/草业科学北京市重点实验室,北京100193

出　　处：《中国草地学报》2024年第12期24-32,共9页Chinese Journal of Grassland

基　　金：乡土草种种子干燥、引发、包衣、丸粒化技术研究(SJCZFY2022-4)。

摘　　要：以老芒麦(Elymus sibiricus)种子为材料,通过控制劣变和PEG引发处理(PEG-5%、PEG-10%和PEG-20%),研究比较未劣变(CK)和劣变14天(D14)种子发芽特性和吸胀过程(未吸胀、6、72和144 h)中抗氧化指标的变化。结果表明,与CK相比,D14种子的发芽能力降低,幼苗生长受到抑制。PEG-10%引发处理显著提高劣变种子的发芽势、发芽率和发芽指数,以及幼苗的根长、苗长、根重和苗重,同时也显著提高了劣变种子在未吸胀时的AsA含量、吸胀6 h时的GSH含量、吸胀72 h时的GR活性和吸胀144 h时的POD活性,降低了吸胀6 h和72 h时的H_(2)O_(2)含量和吸胀144 h时的MDA含量,可以有效保持种子正常的萌发过程。通过聚类分析发现,可以将9个抗氧化防御系统指标分为3组,其中MDA含量、POD和SOD活性在不同吸胀时间的变化趋势一致。In this study,siberian wildrye(Elymus sibiricus)seeds were used as materials to compare the germination characteristics and changes in antioxidant indexes during imbibition process(beginning,6 h,72 h,and 144 h)between non-deteriorated(CK)and deteriorated(D14)seeds under controlled dete‐rioration and polyethylene glycol(PEG)priming treatments(PEG-5%,PEG-10%,and PEG-20%).Results indicated that compared to CK,the seed germination of D14 was reduced and the seedling growth was inhibited.PEG-10%priming significantly enhanced the germination potential,germination percent‐age,germination index,radicle length,seedling length,radicle weight,and seedling weight of deteriorated seeds.Additionally,PEG-10%priming significantly increased the ascorbic acid(AsA)content at begin‐ning of imbibition,glutathione(GSH)content at 6 h of imbibition,glutathione reductase(GR)activity at 72 h of imbibition,and peroxidase(POD)activity at 144 h of imbibition,while reduction of hydrogen per‐oxide(H_(2)O_(2))levels at 6 h and 72 h of imbibition and malondialdehyde(MDA)content at 144 h of imbibi‐tion were observed.Changings on antioxidant indices can effectively maintain the normal germination pro‐cess.In cluster analysis,it was found that the nine antioxidant defense system indicators could be roughly clustered into 3 groups,among which the MDA content,POD activity,and superoxide dismutase(SOD)activity follow the same variation trend at different imbibition times.

关 键 词：老芒麦 种子劣变 PEG引发 抗氧化系统 

分 类 号：S543.9[农业科学—作物学]