一株2,6-二甲基苯酚降解菌的筛选及其降解途径的分析  

Screening of a 2,6-dimethylphenol degrading bacterium and study on its degradation pathway

作　　者：史文婷 向杨 吴菁 闫达中[1] 晁红军 SHI Wenting;XIANG Yang;WU Jing;YAN Dazhong;CHAO Hongjun(School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学生命科学与技术学院,武汉430023

出　　处：《武汉轻工大学学报》2024年第6期36-43,共8页Journal of Wuhan Polytechnic University

基　　金：湖北省科技厅自然科学基金面上项目(编号:2023AFB884);武汉轻工大学校立科研项目(编号:2023Y14).

摘　　要：化合物2,6-二甲基苯酚是一种剧毒和潜在致癌的烷基酚污染物,其降解机制尚不清楚。本文从某污水处理厂的活性污泥中分离到一株降解2,6-二甲基苯酚的菌株26421,鉴定为Pseudomonas sp.。它可以利用2,6-二甲基苯酚、龙胆酸和原儿茶酸,但不能利用邻苯二酚或其他二甲基苯酚异构体作为碳源和能量的来源。Pseudomonas sp.26421在108小时内可以完全降解2.5 mmol/L 2,6-二甲基苯酚。通过HPLC和GC-MS等的鉴定结果表明,该菌能够将2,6-二甲基苯酚的苯环5位羟基化生成2,6-二甲基对苯二酚,然后被进一步转化为2,6-二甲基-ρ-苯醌。这些结果表明,Pseudomonas sp.26421对2,6-二甲基苯酚的降解不是从甲基氧化开始,而是从苯环的羟基氧化开始。The compound 2,6-xylenol is a highly toxic and potential carcinogenic alkylphenol pollutant.However,the mechanisms of its degradation remain unknown.Here,we report the isolation of Pseudomonas sp.26421 from a wastewater treatment factory.It could utilize 2,6-xylenol,gentisate,protocatechuate,but not catechol or other xylenol isomers,as sources of carbon and energy.Pseudomonas sp.26421 completely degraded 2.5 mmol/L 2,6-xylenol within 108 hours.Intact cells of Pseudomonas sp.26421 grew with 2,6-xylenol co-oxidized 2,6-xylenol to 2,6-dimethyl hydroquinone,which was quantitatively converted to 2,6-dimethyl-ρ-benzoquinone that could be accurately identified by HPLC and GC-MS.These results indicate that the degradation of 2,6-xylenol in strain 26421 begins not with the oxidation of methyl group,but from hydroxyl oxidation of the benzene ring.

关 键 词：2 6-二甲基苯酚 微生物降解 分解代谢 Pseudomonas sp.26421 

分 类 号：Q93[生物学—微生物学]