千里光通过抑制肥大细胞P2X7受体缓解炎性疼痛  

Senecio scandens Buch.-Ham alleviates inflammatory pain through inhibiting mast cell P2X7 receptors

作　　者：叶凡[1,2,3] 廖利辉 唐婧玥 陈佳慧 成江 陈功锡 唐宗湘[3] YE Fan;LIAO Li-hui;TANG Jing-yue;CHEN Jia-hui;CHENG Jiang;CHEN Gong-xi;TANG Zong-xiang(School of Pharmaceutical Sciences,Jishou University,Jishou Hunan 416000,China;Key Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College,Jishou Hunan 416000,China;School of Medicine&Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing 210000,China)

机构地区：[1]吉首大学药学院,湖南吉首416000 [2]武陵山区药用资源化学与药理湖南省高校重点实验室,湖南吉首416000 [3]南京中医药大学医学院·整合医学学院,江苏南京210000

出　　处：《中国药理学通报》2025年第1期131-137,共7页Chinese Pharmacological Bulletin

基　　金：湖南省自然科学基金资助项目(No 2024JJ7409);民族药用植物资源与药理重点项目(No 2024KCP001)。

摘　　要：目的探讨千里光对肥大细胞P2X7受体介导的炎症性疼痛的影响。方法采用高浓度ATP诱导脚掌炎症性疼痛模型,利用免疫荧光染色技术,甲苯胺蓝染色技术,探究千里光对肥大细胞上P2X7受体是否有抑制作用。利用钙离子成像实验技术,探究千里光是否可以抑制小鼠腹膜肥大细胞上P2X7受体激活引起的细胞内钙离子富集。利用全细胞膜片钳技术,探究千里光是否可以抑制小鼠腹膜肥大细胞上P2X7受体激活后引起的内向电流。结果体内实验结果显示,千里光(3.9 g·kg^(-1))明显缓解ATP诱导的炎性疼痛(P<0.05);千里光(3.9 g·kg^(-1))明显抑制P2X7受体阳性肥大细胞的浸润(P<0.01);敲除肥大细胞可以消减千里光(3.9 g·kg^(-1))的镇痛效果(P=0.645)。体外实验结果显示,千里光明显抑制肥大细胞P2X7受体介导的钙离子内流(300 mg·L^(-1),P<0.05;1 g·L^(-1),P<0.01;3 g·L^(-1):P<0.01);千里光(1 g·L^(-1))明显抑制肥大细胞P2X7受体介导的内向电流(P<0.01)。结论千里光通过抑制肥大细胞P2X7受体功能缓解ATP诱导的炎性疼痛。Aim To investigate the effect of Senecio scandens Buch.-Ham on inflammatory pain mediated by mast cell P2X7 receptor.Methods Using the ATP-induced foot inflammatory pain,immunofluorescence and toluidine blue staining techniques were used to investigate whether Senecio scandens has inhibitory effect on P2X7 receptor on mast cells.Using the calcium ion imaging experimental technology,to explore whether Senecio scandens inhibit the intracellular calcium ion enrichment caused by activation of P2X7 receptor on mouse peritoneal mast cell.The whole-cell patch clamp technique was used to investigate whether senecio scandens could inhibit the inward current induced by activation of P2X7 receptor on mouse peritoneal mast cell.Results In vivo,Senecio scandens alleviate ATP induced inflammatory pain(3.9 g·kg^(-1):P<0.05),and significantly inhibited the infiltration of P2X7 receptor-positive mast cells(3.9 g·kg^(-1):P<0.05).Knockout of mast cell can reduce the analgesic effect of Senecio scandens(3.9 g·kg^(-1):P=0.645).In vitro.The experiment results show that senecio scandens can significantly inhibit the calcium influx(300 mg·L^(-1):P<0.05;1 g·L^(-1):P<0.01;3 g·L^(-1):P<0.01)and the inward current mediated by P2X7 receptor in mast cell(1 g·L^(-1):P<0.01).Conclusion Senecio scandens alleviate inflammatory pain by inhibiting mast cell P2X7 receptor.

关 键 词：千里光 疼痛 肥大细胞 P2X7受体 炎症 ATP 

分 类 号：R285[医药卫生—中药学]