升降散改善MNNG诱导的胃黏膜损伤及其机制  

The effects and mechanisms about Shengjiang powder in improving MNNG-induced gastric mucosal injury

作　　者：刘轶璜 张思佳 刘菲 陈苹苹 LIU Yi-huang;ZHANG Si-jia;LIU Fei;CHEN Ping-ping(College of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050091,China;Hebei International Cooperation Center for lon Channel Function and Innovative Traditional Chinese Medicine,Shijiazhuang 050091,china;College of Integrative Chinese and Western Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050091,China)

机构地区：[1]河北中医药大学药学院,河北石家庄050091 [2]河北省离子通道功能与创新中药国际联合研究中心,河北石家庄050091 [3]河北中医药大学中西医结合学院,河北石家庄050091

出　　处：《中国药理学通报》2025年第1期178-185,共8页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年项目(No 82003990);河北省自然科学基金中医药联合基金培育项目(No H2022423364);河北省中医药管理局科研计划项目(No 2024087);河北省自然科学基金面上项目(No H2020423252)。

摘　　要：目的确定升降散对胃黏膜损伤的改善作用并探讨其机制。方法通过文献计量学研究了解升降散的研究现状。采用液质联用技术获取升降散主要药效成分,通过网络药理学分析预测机制。对胃黏膜损伤大鼠灌胃给予阳性药及不同剂量升降散,通过HE染色,观察胃组织形态。ELISA实验检测血清中TNF-α、IL-1β的水平。IHC实验评价胃黏膜MUC5AC、MUC6的表达。结果文献计量学分析表明,升降散在内外伤杂病治疗方面应用广泛。根据升降散主要药效成分中浓度前20位的化合物得到的作用靶点525个,与胃炎相关靶点相交集的129个;GO分析得条目数2127条,KEGG分析得通路140条;说明升降散具有多靶点多通路改善胃黏膜损伤的可能性。HE染色结果显示,升降散确能改善大鼠胃黏膜炎症。ELISA实验结果显示,升降散可有效下调大鼠血清中的TNF-α和IL-1β。IHC实验结果表明,升降散有效下调MUC5AC的表达、上调MUC6的表达。结论升降散能以缓解炎症、调节表面黏液屏障的方式改善胃黏膜损伤。Aim To determine the effects of Shengjiang Powder on gastric mucosal injury and explore its mechanisms.Methods A bibliometric study was conducted to understand the current research status of Shengjiang Powder.The main pharmacological components of Shengjiang Powder were obtained using liquid chromatography-mass spectrometry(LC-MS),and mechanisms were predicted through network pharmacology analysis.Sprague-Dawley Rats with gastric mucosal injury were treated with Vatacoenayme and different doses of Shengjiang Powder by intragastric administration.Gastric tissues were collected and stained with HE to observe morphological changes.ELISA was used to detect the levels of TNF-αand IL-1βin serum.IHC was used to evaluate the expression of MUC5AC and MUC6 in the gastric mucosa.Results Bibliometric analysis indicated that Shengjiang Powder is widely used in the treatment of various internal and external injuries.LC-MS identified the top 20 compounds as the main pharmacological components of Shengjiang Powder,yielding 525 target compounds,of which 129 overlapped with gastritis-related targets.GO analysis identified 2,127 entries,and KEGG analysis identified 140 pathways,suggesting that Shengjiang Powder might improve gastric mucosal injury through multiple targets and pathways.HE staining results demonstrated that Shengjiang Powder could significantly improve gastric mucosal inflammation in rats.ELISA results showed that Shengjiang Powder effectively reduced the levels of TNF-αand IL-1βin rat serum.IHC results indicated that Shengjiang Powder effectively downregulated MUC5AC expression and upregulated MUC6 expression.Conclusion Shengjiang Powder can improve gastric mucosal injury by alleviating inflammation and regulating the surface mucus barrier.

关 键 词：升降散 慢性胃炎 文献计量学 网络药理学 胃组织结构 炎症因子 免疫组织化学 

分 类 号：R285.5[医药卫生—中药学]