酸柏栀油软胶囊对肥胖大鼠血脂及GSH、MDA水平的影响  

The effect of blood lipids,glutathione and malondialdehyde in SD rats by action of Soft Capsule of Oil of Jujube

作　　者：张玉桐 党韵涵 赵忻未 王嘉琪 张诗敏 曹朵 王磊[4] 张正祥[1] ZHANG Yutong;DANG Yunhan;ZHAO Xinwei;WANG Jiaqi;ZHANG Shimin;CAO Duo;WANG Lei;ZHANG Zhengxiang(Yan'an Medical College of Yan'an University Yan'an 716000,China;College of History and Culture,Yan'an University,Yan'an 716000,China;College of Life and Sciences,Yan'an University,Yan'an 716000,China;Affiliated Hospital of Yan'an University,Yan'an 716000,China)

机构地区：[1]延安大学延安医学院,陕西延安716000 [2]延安大学历史文化学院,陕西延安716000 [3]延安大学生命科学学院,陕西延安716000 [4]延安大学附属医院,陕西延安716000

出　　处：《延安大学学报（医学科学版）》2024年第4期15-18,29,共5页Journal of Yan'an University:Medical Science Edition

基　　金：陕西省自然科学基础研究计划项目(2022JM-607);延安市科研计划项目(2023-CYL-109-21)。

摘　　要：目的探究酸柏栀油软胶囊(soft capsule of compound oil of jujube,arboruitae,and gardenia,COJAG)对肥胖大鼠血脂水平及谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)等氧化应激指标的影响。方法选取48只SD大鼠,雌雄对半,按照体重随机分为空白对照组、模型对照组、辛伐他汀组(0.3 mg/100 g)、COJAG低剂量组(0.072 g/100 g)、COJAG中剂量组(0.18 g/100 g)、COJAG高剂量组(0.45 g/100 g)。空白对照组给予普通饲料,其余各组给予高脂饲料,同时各给药组灌胃对应剂量的药物。5周后,腹腔注射戊巴比妥钠麻醉大鼠,采用摘除眼球取血法,取血后分离血清,检测SD大鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(total glyceride,TG)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)及GSH、MDA水平。结果模型对照组大鼠高脂饮食后体质量增加,血清TC、TG和LDL-C水平升高,HDL-C水平下降,GSH水平下降,与空白对照组相比,差异有统计学意义(P<0.05)。COJAG各剂量组大鼠药物干预后体质量均上升,血清TC、TG和LDL-C水平均下降,HDL-C水平均有所升高,GSH水平显著上升,MDA水平显著下降,与模型对照组相比,差异均具有统计学意义(P<0.05)。结论COJAG可降低高脂饮食诱导的肥胖大鼠血脂水平,升高其体内谷胱甘肽和高密度脂蛋白胆固醇水平,并降低其体内丙二醛水平,具有抗氧化能力。Objective To investigate the oxidative stress indicators effects of soft capsule of compound oil of jujube,arboruitae,and gardenia(COJAG)on blood lipid levels and glutathione(GSH)and malondialdehyde(MDA)in obese rats.Methods 48 SD rats,half male and half female,were selected and randomly divided into blank control group,model control group,simvastatin group(0.3 mg/100 g),low dose group of COJAG(0.072 g/100 g),middle dose group of COJAG(0.18 g/100 g),high dose group of COJAG(0.45 g/100 g)according to their body weights.Blank control group give ordinary feed,the rest of the groups give high fat feed,at the same time each drug administration group gavage corresponding dose of drugs.Five weeks later,rats were anesthetized with pentobarbital sodium via intraperitoneal injection.The enucleation of the eyeball was used for blood collection,and the serum was separated to detect total cholesterol(TC),total triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),and GSH、MDA in the serum of SD rats.Results After a high-fat diet,the body mass of the model control group rats increased,serum TC,TG,and LDL-C levels increased,HDL-C levels decreased,and GSH levels decreased.Compared with the blank control group,the differences were statistically significant(P<0.05).After drug intervention,the body mass of rats in each dose group of COJAG increased,serum TC,TG,and LDL-C levels decreased,HDL-C levels increased,GSH levels significantly increased,and MDA levels significantly decreased.Compared with the model control group,the differences were statistically significant(P<0.05).Conclusion COJAG can reduce the blood lipid level of high-fat diet-induced obese rats,elevate the levels of glutathione and high-density lipoprotein cholesterol in their bodies,and reduce the level of malondialdehyde in their bodies,with antioxidant capacity.

关 键 词：酸柏栀油软胶囊 肥胖 血脂 抗氧化 氧化应激 

分 类 号：R965[医药卫生—药理学]