海金沙幼孢子体生根壮苗条件的优化研究  

Optimization of Rooting and Seedling Growth Conditions for Young Sporophytes of Lygodium japonicum

作　　者：刘薇 童家赟[1] 詹若挺[1,2] 刘赛 LIU Wei;TONG Jia-yun;ZHAN Ruo-ting;LIU Sai(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Key Laboratory of Chinese Medicinal Resource from Lingnan(Guangzhou University of Chinese Medicine),Ministry of Education,Guangzhou 510006,China;Dongguan Asian Pharmaceutical Technology Co.,Ltd.,Dongguan 523160,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]岭南中药资源教育部重点实验室(广州中医药大学),广东广州510006 [3]东莞市亚洲制药科技有限公司,广东东莞523160

出　　处：《江西农业学报》2024年第12期31-39,共9页Acta Agriculturae Jiangxi

基　　金：广东省省级乡村振兴战略专项资金种业振兴项目(2022-NJS-00-002、粤财农[2022]184号);东莞市科技特派员项目。

摘　　要：为了筛选蕨类植物海金沙幼孢子体生根壮苗的最佳培养条件以获得优质的种苗,以无菌萌发培养得到的海金沙幼孢子体为材料,考察了不同培养密度(1、3、5、7个/瓶)、培养基无机盐(1/4MS、1/2MS、MS)、蔗糖(1%、2%、3%)、外源激素NAA和IBA(浓度0.1、0.3、0.5 mg/L、单因素或两两组合)对海金沙幼孢子体生根壮苗的影响。结果表明:培养密度为5~7个/瓶较为适宜,幼孢子体生长良好;幼孢子体生根壮苗的最佳培养基1/4MS+1%蔗糖+0.3 mg/L IBA+0.3 mg/L NAA,能显著提高幼孢子体的幼苗鲜重和干重;在培养90 d左右即可炼苗移栽,此时幼孢子体叶片较大、叶色较深、根系发育良好,移栽不宜太迟,否则会导致其营养不足,叶片发黄和枯萎。In order to screen the optimal culture conditions for young sporophyte rooting and seedling growth of Lygodium japonicum,and obtain high-quality seedlings,young sporophytes from aseptic germination culture were used as the explants,and the effects of different culture densities(1,3,5 and 7 sporophytes/flask),different concentrations of inorganic salt in culture medium(1/4MS,1/2MS and MS),also sucrose(1%,2% and 3% of concentrations),exogenous hormones NAA and IBA(0.1,0.3 and 0.5 mg/L used singly or in pairs on rooting and seedling growth of young sporophytes were investigated.The results showed that the culture density of 5~7 sporophytes per flask was more suitable,and the young sporophytes grew well.The optimal culture medium for sporophyte rooting and seedling growth was 1/4MS+1% sucrose+0.3 mg/L IBA+0.3 mg/L NAA,which could significantly improve the fresh and dry weight of sporophytes.Transplanting should be carried out after cultivating for about 90 days,while the sporophyte leaves are larger and deeper green,and the root system is good.It should not be too late,otherwise sporophyte would be under nutrition-deficient,and the leaves would turn yellow and wilt.

关 键 词：海金沙 孢子体 无菌培养 生根壮苗 

分 类 号：Q914.85[天文地球—古生物学与地层学]