硫酸铜对河川沙塘鳢的急性毒性、肝脏抗氧化酶活性及组织结构的影响  

Effects of CuSO4 on Acute Toxicity,Liver Antioxidant Enzyme Activity and Tissue Structure of Odontobutis potamophila

作　　者：刘国兴[1,2] 李杰 周梓涵 郑友 丁淑燕[1,2] 郝忱 蒋琦辰[1,2] LIU Guo-xing;LI Jie;ZHOU Zi-han;ZHENG You;DING Shu-yan;HAO Chen;JIANG Qi-chen(Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017,China;Low-temperature Germplasm Bank of Important Economic Fish of Jiangsu Provincial Science and Technology Resources(Agricultural Germplasm Resources)Coordination Service Platform,Nanjing 210017,China)

机构地区：[1]江苏省淡水水产研究所,江苏南京210017 [2]江苏省科技资源(农业种质)统筹服务平台重要经济鱼类低温种质库,江苏南京210017

出　　处：《江西农业学报》2024年第12期65-72,92,共9页Acta Agriculturae Jiangxi

基　　金：江苏现代农业产业技术体系建设项目(JATS[2023]376、JATS[2023]378)。

摘　　要：为探究硫酸铜(CuSO_(4))对河川沙塘鳢(Odontobutis potamophila)的急性毒性效应,采用静水生物测试法,根据预试验结果,设定了1.58、2.22、3.16、6.33、12.66和15.82 mg/L共6个浓度梯度进行急性毒性试验,并设定了0.50、0.75、1.00和1.25 mg/L共4个浓度梯度进行急性暴露试验,分别在6、12、24、48、72、96 h时测定肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)含量和肝、鳃组织结构的变化。结果表明:CuSO_(4)对河川沙塘鳢24、48、72和96 h的LC_(50)分别为8.38、3.87、2.92和2.50 mg/L,SC为0.25 mg/L。随着时间的推移和CuSO_(4)浓度的升高,SOD、CAT和GSH-PX活性呈现出先上升后下降的趋势,达峰值时与CK差异显著;MDA含量呈现出先下降后上升的趋势,在最低值和峰值时均与CK差异显著。急性暴露后的河川沙塘鳢肝组织出现空泡化、坏死,细胞核溶解消失,鳃组织出现肿胀变形、坏死脱落。因此,SOD、CAT、GSH-PX活性和MDA含量以及肝、鳃组织结构的变化均反映了河川沙塘鳢机体的受损伤程度,可作为后续河川沙塘鳢养殖试验中用药评价的参考标准。In order to explore the acute toxicity effect of copper sulfate(CuSO_(4))on Odontobutis potamophila,the static biological test method was used to set six concentration gradients of 1.58,2.22,3.16,6.33,12.66 and 15.82 mg/L according to the preliminary experimental results.According to the results of acute toxicity test,four concentration gradients of 0.50,0.75,1.00 and 1.25 mg/L were set for acute exposure test.The activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX)and malondialdehyde(MDA)content in liver tissue and the changes of liver and gill tissue structure were measured at 6,12,24,48,72 and 96 h,respectively.The results showed that the LC_(50)of CuSO_(4)to O.potamophila at 24,48,72 and 96 h were 8.38,3.87,2.92 and 2.50 mg/L,respectively,and the safe concentration was 0.25 mg/L.With the increase of time and CuSO_(4)concentration,the activities of SOD,CAT and GSH-PX increased first and then decreased,and the difference was significant compared with the control group at the peak value.The MDA content showed a trend of decreasing first and then increasing,and the difference was significant compared with the control group at the lowest and peak values.After acute exposure,the liver tissue of O.potamophila showed vacuolization and necrosis,the nucleus dissolved and disappeared,and the gill tissue showed swelling and degeneration,necrosis and shedding.The above results showed that the changes of SOD,CAT,GSH-PX activity,MDA content,and liver and gill tissue structure reflected the degree of damage to the body of O.potamophila,which could be used as a reference for safety risk assessent of O.potamophila breeding in the future.

关 键 词：河川沙塘鳢 硫酸铜 安全浓度 组织结构 抗氧化酶 

分 类 号：S943[农业科学—水产养殖]