环状RNA circ-SLAIN2在急性心肌炎患者血清中表达及对心肌细胞活性的影响  

Expression of circular RNA circ-SLAIN2 in serum of patientswith acute myocarditis and its effect on myocardial cell activity

作　　者：朱丽 伍仙凤 夏玮 刘玲玲 徐薇 ZHU Li;WU Xianfeng;XIA Wei;LIU Lingling;XU Wei(Department of Cardiovascular Medicine,Jingmen People’s Hospital,Jingmen 448000,China)

机构地区：[1]荆门市人民医院荆楚理工学院附属中心医院心血管内科,湖北荆门448000 [2]荆门市人民医院荆楚理工学院附属中心医院老年科,湖北荆门448000 [3]苏州大学附属第二医院心血管内科,江苏苏州215004

出　　处：《陕西医学杂志》2025年第1期21-26,共6页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(31870903)。

摘　　要：目的:观察环状RNA(circRNA)circ-SLAIN2在急性心肌炎患者血清中的表达,探讨circ-SLAIN2对心肌细胞增殖和凋亡的影响及作用机制。方法:收集35例急性心肌炎患者血清标本,另选取35例体检健康者为对照,采用实时定量聚合酶链反应(qRT-PCR)检测血清中circ-SLAIN2表达量。选取人心肌细胞系HCM,分别感染空载慢病毒(对照组)或载有circ-SLAIN2序列的慢病毒(circ-SLAIN2组)。qRT-PCR检测circ-SLAIN2过表达效率。分别采用脂多糖诱导心肌炎,采用细胞计数试剂盒8(CCK-8)法和流式细胞术检测过表达circ-SLAIN2对人心肌细胞增殖和凋亡的影响。LncBase v.2、Linc2GO、LNCipedia、miRcode在线数据库和双荧光素酶报告基因实验分析circ-SLAIN2和微小RNA-429(miR-429)的靶向关系。qRT-PCR检测miR-429表达水平。蛋白质印迹(Western blot)法检测丝裂原活化蛋白激酶p38(p38MAPK)/核因子κB(NF-κB)信号通路蛋白和凋亡相关蛋白的表达水平。结果:急性心肌炎患者血清中circ-SLAIN2表达较体检健康者显著降低(P<0.01)。与对照组比较,circ-SLAIN2组HCM细胞中circ-SLAIN2表达显著上升(P<0.01)。经过脂多糖处理36 h后,circ-SLAIN2组HCM细胞增殖吸光度明显高于对照组(P<0.01),circ-SLAIN2组HCM细胞凋亡率明显低于对照组(P<0.01)。circ-SLAIN2能够靶向结合miR-429。与对照组比较,circ-SLAIN2组HCM细胞中miR-429表达显著降低(P<0.01),p38MAPK/NF-κB信号通路蛋白表达显著降低(P<0.01),细胞凋亡蛋白Bcl-2相关X蛋白(Bax)、Bcl-2相关K蛋白(Bak)表达量降低(均P<0.01)。结论:circ-SLAIN2在急性心肌炎患者血清中低表达,过表达circ-SLAIN2通过负向调节miR-429表达,增强心肌细胞的活性并抑制细胞的凋亡。Objective:To observe the expression of circular RNA(circRNA)circ-SLAIN2 in serum of patients with acute myocarditis,and to explore the effect of circ-SLAIN2 on myocardial cell proliferation and apoptosis and its mechanism.Methods:Serum samples from 35 patients with acute myocarditis treated in the Department of Cardiovascular Medicine,Jingmen People’s Hospital from July 2020 to May 2023 were collected,and 35 healthy individuals were selected as controls.The expression of circ-SLAIN2 in serum was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The human cardiomyocyte cell line HCM was selected and infected with empty lentivirus(Control group)or lentivirus loaded with circ-SLAIN2 sequence(circ-SLAIN2 group).The overexpression efficiency of circ-SLAIN2 was detected by qRT-PCR.Myocarditis was induced by lipopolysaccharide,and the effects of circ-SLAIN2 overexpression on the proliferation and apoptosis of human cardiomyocytes were detected by Cell Counting Kit-8(CCK-8)assay and flow cytometry.The LncBase v.2,Linc2GO,LNCipedia,miRcode online database and dual-luciferase reporter gene experiments were used to analyze the targeting relationship between circ-SLAIN2 and miR-429.The expression level of miR-429 was detected by qRT-PCR.Western blot was used to detect the expression levels of Mitogen-activated protein kinase p38(p38MAPK)/nuclear factor-kappa B(NF-κB)signaling pathway proteins and apoptosis-related proteins.Results:The expression of circ-SLAIN2 in serum of patients with acute myocarditis was significantly lower than that of healthy subjects(P<0.01).Compared with the control group,the expression of circ-SLAIN2 in HCM cells in the circ-SLAIN2 group was significantly increased(P<0.01).After lipopolysaccharide treatment for 36 h,the absorbance of HCM cell proliferation in circ-SLAIN2 group was significantly higher than that in Control group(P<0.01),and the apoptosis rate of HCM cells in circ-SLAIN2 group was significantly lower than that in Control group(P<0.01).circ-SLAIN2 can target and b

关 键 词：急性心肌炎 环状RNA circ-SLAIN2 微小RNA-429 细胞增殖 细胞凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]