白藜芦醇对实验性自身免疫性心肌炎大鼠心肌巨噬细胞极化及心肌炎性损伤的影响  

The influence of resveratrol on macrophage polarization and myocardial inflammatoryinjury in rats with experimental autoimmune myocarditis

作　　者：林琳[1] 樊刚 李青[1] 朱文静[1] 薛嘉虹 魏瑾[1] LIN Lin;FAN Gang;LI Qing;ZHU Wenjing;XUE Jiahong;WEI Jin(Department of Cardiology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)

机构地区：[1]西安交通大学第二附属医院心血管病院,陕西西安710004 [2]陕西中医药大学附属咸阳市中心医院,陕西咸阳712000

出　　处：《陕西医学杂志》2025年第1期27-32,共6页Shaanxi Medical Journal

基　　金：陕西省重点研发计划项目(2024SF-YBXM-005)。

摘　　要：目的:探讨白藜芦醇(Res)对实验性自身免疫性心肌炎(EAM)大鼠心肌巨噬细胞极化及炎性损伤的影响。方法:建立EAM大鼠模型,分为对照组、EAM组、Resveratrol组,通过免疫荧光、HE染色观察心肌组织巨噬细胞浸润程度并对心肌病理损伤进行评分;采用Realtime PCR检测巨噬细胞极化标记分子诱生型一氧化氮合酶(iNOS)、精氨酸酶1(Arg1)及炎症因子白细胞介素6(IL-6)、白细胞介素1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的表达变化,Western blot检测NLRP3炎性小体蛋白组分的表达变化。结果:EAM组大鼠心肌组织可见大量巨噬细胞浸润,伴有明显的心肌纤维断裂、溶解;IL-6、IL-1β、TNF-α、iNOS mRNA表达均较Control组显著增加(分别为P<0.05,P<0.05,P<0.01,P<0.01),而Arg1表达较Control组显著减少(P<0.05);Resveratrol可减少EAM大鼠心肌组织巨噬细胞浸润数量及心肌病理损伤积分(均P<0.05),减少IL-6、IL-1β、TNF-α、iNOS mRNA的表达(均P<0.05),增加Arg1的表达(P<0.01)。同时,与Control组比较,EAM大鼠心肌组织NLRP3和Caspase-p10、pro-Caspase-1及IL-1β蛋白表达明显升高(均P<0.05),Resveratrol可显著减少人NOD样受体家组蛋白3(NLRP3)、Caspase-p10、pro-Caspase-1及IL-1β蛋白表达,差异具有统计学意义(均P<0.05)。结论:巨噬细胞浸润并向M1型极化参与EAM大鼠心肌炎性损伤;Res可能通过抑制M1型巨噬细胞向M2型极化、NLRP3炎症小体激活及相关炎性因子释放减轻EAM心肌损伤。Objective:To explore the effects of resveratrol on myocardial macrophage polarization and inflammatory injury in experimental autoimmune myocarditis(EAM)rats.Methods:Experimental myocarditis model was established and randomly divided into 3 groups:Control group(Control),EAM model group(EAM)and Resveratrol treatment group(Resveatrol).The degree of macrophage infiltration were detected by immunofluorescence and the degree of myocardial injury was detected by HE stain.The expression of markers(iNOS and Arg1)of M1 and M2 macrophage and inflammatory factors were detected by Realtime PCR.Western blot was used to detect the expression changes of NLRP3 inflammasome protein components.Results:Large numbers of macrophages were observed infiltrating the myocardial tissue of EAM group rats,accompanied by significant myocardial fiber rupture and dissolution;The mRNA expression of IL-6,IL-1β,TNF-α,and iNOS were significantly increased in the control group compared to the control group(P<0.05,P<0.05,P<0.01,P<0.01),The expression of Arg1 was significantly reduced compared to the control group(P<0.05),and the difference was statistically significant;Resveratrol can reduce the infiltration of macrophages and myocardial pathological injury score in EAM rats’myocardial tissue(P<0.05),decrease the expression of IL-6,IL-1β,TNF-α,iNOS mRNA(P<0.05),and increase the expression of Arg1(P<0.01),with statistical significance.Meanwhile,compared with the Control group,the protein expression of NLRP3 and Caspase-p10,pro-Caspase-1,and IL-1βin the myocardial tissue of EAM rats was significantly increased.Resveratrol significantly reduced the protein expression of NLRP3,Caspase-p10,pro-Caspase-1,and IL-1β(all P<0.05),and the difference was statistically significant.Conclusion:Macrophage infiltration and polarization towards M1 type are associated with myocardial injury in EAM rats;Res may alleviate EAM myocardial injury by inhibiting polarization of M1 macrophages towards M2,activation of NLRP3 inflammasomes,and reduced release of inflamm

关 键 词：巨噬细胞极化 炎症因子 白藜芦醇 实验性自身免疫性心肌炎 心肌损伤 人NOD样受体家组蛋白3炎症小体 

分 类 号：R542.2[医药卫生—心血管疾病]