人参皂苷通过JAK激酶/信号转导和转录激活因子信号通路抑制慢性心衰大鼠心肌自噬的机制研究  

The mechanism of ginsenosides inhibiting myocardial autophagyin chronic heart failure rats through JAK/STAT signaling pathway

作　　者：韩萌 李玉洁 杨国宁 胡辉 林正捷 陈原林 HAN Meng;LI Yujie;YANG Guoning;HU Hui;LIN Zhengjie;CHEN Yuanlin(Department of Cardiology,Affiliated Hospital of Xingtai Medical College,Xingtai 054000,China)

机构地区：[1]邢台医学院附属医院心内科,河北邢台054000 [2]邢台市中心医院心内科,河北邢台054000

出　　处：《陕西医学杂志》2025年第1期33-37,共5页Shaanxi Medical Journal

基　　金：河北省中医药管理局科研计划项目(2021453);河北省邢台市科技计划项目(2023zc067)。

摘　　要：目的:探讨人参皂苷通过Janus激酶/信号转导和转录激活因子(JAK/STAT)信号通路抑制慢性心力心衰(CHF)大鼠心肌自噬的机制研究。方法:选取65只雄性SD大鼠,随机分为对照组、模型组、人参皂苷Rb1低、中、高剂量组,建立大鼠CHF模型,排除死亡大鼠后,每组12只。对照组、模型组以10 ml/kg蒸馏水灌胃,其余三组分别以30、50、70 mg/kg人参皂苷Rb1灌胃,1次/d,连续给药8周。以超声仪器测各组大鼠心功能及血流动力学,以苏木精-伊红(HE)染色和TUNEL法分别测各组大鼠观察心肌组织结构及细胞凋亡情况,蛋白质免疫印迹(Western blot)检测心肌组织中JAK/STAT通路及自噬效应蛋白(Beclin1)、微管相关蛋白1轻链(LC3-Ⅱ)蛋白表达。结果:模型组与对照组比较,心功能、血流动力学下降,人参皂苷Rb1低、中、高剂量组与模型组比较,心功能、血流动力学提升(均P<0.05)。模型组心肌组织结构不完整,细胞肿胀变形,界限模糊,间隙增大,可见脱落的细胞核,并有少量炎性细胞浸润。人参皂苷Rb1低、中、高剂量组心肌结构、细胞界限和大小及排列形态逐渐改善,好于模型组。对照组、模型组、人参皂苷Rb1低、中、高剂量组凋亡率依次为(3.12±1.01)%、(34.04±8.76)%、(28.06±7.24)%、(18.05±5.13)%、(15.17±4.52)%(均P<0.05)。模型组与对照组比较,心肌组织JAK1、STAT3、Beclin1、LC3-Ⅱ蛋白表达升高,人参皂苷Rb1低、中、高剂量组与模型组比较,心肌组织JAK1、STAT3、Beclin1、LC3-Ⅱ蛋白表达依次降低(均P<0.05)。结论:人参皂苷Rb1可保护CHF大鼠心功能,调节心肌细胞自噬,其机制可能与抑制JAK/STAT信号通路有关。Objective:To investigate the mechanism of ginsenosides inhibiting myocardial autophagy in rats with chronic heart failure(CHF)through Janus kinase/signal transduction and transcriptional activator(JAK/STAT).Methods:65 male SD rats were randomly divided into control group,model group,ginsenoside Rb1 low-dose,medium-dose and high-dose groups.Rat CHF model was established,and 12 rats in each group were excluded.The control group and model group were given 10 ml/kg distilled water intragastric administration,and the other three groups were given 30 mg/kg,50 mg/kg and 70 mg/kg ginsenoside Rb1 intragastric administration once a day for 8 weeks.The cardiac function and hemodynamics of rats in each group were measured by ultrasound,and the myocardial tissue structure and cell apoptosis were measured by hematoxylin-eosin(HE)staining and TUNEL method.The expression of JAK/STAT pathway and Beclin1,microtubule-associated protein 1 light chain(LC3-Ⅱ)protein in myocardial tissue was detected by Western Blot.Results:Compared with the control group,the heart function and hemodynamics of the model group were decreased,and the heart function and hemodynamics of the ginsenoside Rb1 low,medium and high dose groups were increased compared with the model group(均P<0.05).In the model group,the myocardial tissue structure was incomplete,the cells were swollen and deformed,the boundary was blurred,the space was enlarged,the nucleus was shed,and a small amount of inflammatory cells were infiltrated.Ginsenoside Rb1 The structure,cell boundary,size and arrangement of myocardium in low,medium and high dose ginsenoside Rb1 groups were gradually improved,which was better than that in model group.The apoptosis rates in control group,model group,ginsenoside Rb1 low,medium and high dose groups were(3.12±1.01)%,(34.04±8.76)%,(28.06±7.24)%,(18.05±5.13)%,(15.17±4.52)%(all P<0.05).Compared with the control group,the protein expressions of JAK1,STAT3,Beclin1 and LC3-Ⅱin the myocardium of the model group were increased,while the protein expre

关 键 词：慢性心力衰竭 人参皂苷RB1 JAK激酶/信号转导和转录激活因子信号通路 心肌细胞 心功能 自噬 

分 类 号：R541.6[医药卫生—心血管疾病]