解毒活血汤对慢性肾脏病大鼠肾脏Smad2、Smad3表达的影响  

Effects of Jiedu Huoxue decoction on CKD rat kidney Smad2,Smad3

作　　者：杨灡 孙妲男[2] 李勇 徐丽 周鹏 代璇 侯兴 褚宇鹏 李敬槟 YANG Lan;SUN Danan;LI Yong;XU Li;ZHOU Peng;DAI Xuan;HOU Xing;CHU Yupeng;LI Jingbing(Graduate School of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学研究生院,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第二医院肾内科,黑龙江哈尔滨150001

出　　处：《陕西中医》2025年第1期19-24,共6页Shaanxi Journal of Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金资助项目(LH2021H086);黑龙江省中医药科研项目(ZHY2020-139,ZHY2022-176);黑龙江省博士后研究人员落户黑龙江科研启动金资助项目(LBH-Q21190)。

摘　　要：目的:观察解毒活血汤通过调控慢性肾脏病(CKD)大鼠Smads蛋白在肾脏组织中的表达对CKD的作用。方法:将72只雄性SD大鼠随机分成六组,空白组、模型组、阳性对照组,中药低剂量组、中药中剂量组以及中药高剂量组,每组各12只。除空白组外,其余组的大鼠均通过腺嘌呤悬浮液灌胃并饲喂高磷饮食以建立CKD大鼠模型,造模8周后开始对大鼠进行为期12周的药物灌胃处理,在第12周末,对大鼠血液样本进行肌酐(Scr)、尿素氮(BUN)、磷(P)和血钙(Ca)的生化检测,并用免疫组化法分析肾组织中Smad2、Smad3蛋白的表达。结果:与空白组相比,其余组大鼠血清中的Scr、BUN、P和Ca含量均有显著性差异(P<0.01或P<0.05);与模型组相比,其余组大鼠血清中的Scr、BUN和P的含量普遍降低(P<0.05或P<0.05),而Ca含量则有所上升(P<0.05);与空白组相比,其余组大鼠肾组织中Smad2和Smad3的表达显著增加(P<0.05)。与模型组相比,其余组肾组织中Smad2和Smad3的表达均有所降低(P<0.05)。相较于解毒活血汤低剂量组,高剂量组、中剂量组及阳性对照组的Smad2蛋白和Smad3蛋白表达量均进一步下降(P<0.05)。结论:解毒活血汤显示出对延缓CKD进程的积极作用,并能有效升高肾组织中Smad2、降低Smad3的表达。相较于其余用药组,解毒活血汤高剂量组展现出更显著的疗效,药物剂量与疗效之间存在正相关关系。Objective:To observe the effect of Jiedu Huoxue decoction on chronic kidney disease(CKD)by regulating the expression of Smads protein in kidney tissues of rats with CKD.Methods:Seventy-two male SD rats were randomly divided into six groups,the blank group,the model group,the positive control group,the low-dose group of traditional Chinese medicine,the medium-dose group of traditional Chinese medicine,and the high-dose group of traditional Chinese medicine,with 12 rats in each group.Except for the blank group,the rats in the other groups were gavaged with adenine suspension and fed a high-phosphorus diet to establish a CKD rat model,and the rats were treated with the drug by gavage for a period of 12 weeks after the modeling period of 8 weeks.On the 12th weekend,biochemical tests were conducted on rat blood samples for creatinine(Scr),urea nitrogen(BUN),phosphorus(P),blood calcium(Ca).On the 12th weekend,expression of Smad2 and Smad3 proteins in the kidney tissues.Results:Comparison of data between groups was made after completing the whole process of drug administration and analysis.Compared with the blank group,the serum levels of Scr,BUN,P and Ca in the remaining groups of rats were significantly different(P<0.01 or P<0.05);compared with the model group,the serum levels of Scr,BUN and P in the remaining groups of rats were generally decreased(P<0.05 or P<0.05),while Ca levels were increased(P<0.05).Compared with the blank group,the remaining groups of The expression of Smad2 and Smad3 in rat kidney tissues was significantly increased(P<0.05).Compared with the model group,the expression of Smad2 and Smad3 in the kidney tissues of the rest of the groups were decreased(P<0.05).Compared with the low-dose group of detoxification and activation of blood circulation soup,the expression of Smad2 protein and Smad3 protein in the high-dose group,middle-dose group and positive control group were further decreased(P<0.05).Conclusion:Jiedu Huoxue decoction shows a positive effect on delaying the process of CKD and effective

关 键 词：慢性肾脏病 解毒活血汤 大鼠 去宛陈莝 免疫组化法 SMAD2 SMAD3 

分 类 号：R692[医药卫生—泌尿科学]