胶艾汤对模拟特发性流产的线粒体调控作用机制  

The mechanism of mitochondrial regulation by Jiao Ai decoction in a model of idiopathic miscarriage

作　　者：陈西 罗岚[1] 宁东红[1] 谢春红[1] 彭艳丽[1] 梁煦 CHEN Xi;LUO Lan;NING Donghong;XIE Chunhong;PENG Yanli;LIANG Xu(Department of Traditional Chinese Medicine Gynecology,Hunan Maternal and Child Health Hospital,Changsha 410000,China)

机构地区：[1]湖南省妇幼保健院中医妇科,湖南长沙410000

出　　处：《陕西中医》2025年第1期31-37,共7页Shaanxi Journal of Traditional Chinese Medicine

基　　金：湖南省中医药科研计划项目(B2024107)。

摘　　要：目的:探究胶艾汤对模拟特发性流产的线粒体调控作用机制。方法:细胞实验设置对照组、胶艾汤组、miR-29α-3p模拟物组、胶艾汤+miR-29α-3p模拟物组,对人绒毛膜滋养层细胞HTR-8/SVneo进行培养和处理,检测miR-29α-3p表达、线粒体凋亡、细胞色素C释放、凋亡相关蛋白表达、细胞迁移和侵袭能力。动物实验选用怀孕的C57BL/6J小鼠建立流产模型,分组进行胶艾汤灌胃治疗和miR-29α-3p模拟物腹腔注射治疗,观察流产情况、胎盘组织学变化、miR-29α-3p表达、线粒体凋亡相关指标。结果:细胞实验中,与对照组相比,胶艾汤组里miR-29α-3p的表达水平于48 h明显提升(P<0.001),miR-29α-3p模拟物组的表达水平也显著高于对照组,而胶艾汤+miR-29α-3p模拟物组的表达水平最高。胶艾汤和miR-29α-3p模拟物能够降低线粒体膜电位变化,减少细胞色素C的释放,调节凋亡相关蛋白(Bax表达降低,Bcl-2表达升高,Caspase-3活性降低)的表达,使其向抗凋亡方向转变,从而抑制线粒体凋亡,联合使用时效果更优。胶艾汤和miR-29α-3p模拟物还能够促进细胞的迁移和侵袭能力,联合使用时效果更为显著。动物实验中,流产模型组的流产率较高,为(31.17±0.67)%;胶艾汤治疗组的流产率降低至(13.30±0.36)%;胶艾汤+miR-29α-3p模拟物组的流产率进一步降低至(5.77±0.36)%(P<0.001)。胶艾汤治疗组胎盘组织的形态和结构得到改善,滋养层细胞的损伤减轻;胶艾汤+miR-29α-3p模拟物组胎盘组织的损伤程度更轻,滋养层细胞形态基本正常。胶艾汤治疗组与胶艾汤+miR-29α-3p模拟物组在胎盘组织中miR-29α-3p的表达水平提升,而且胶艾汤+miR-29α-3p模拟物组的表达水平更高。胶艾汤治疗组和胶艾汤+miR-29α-3p模拟物组胎盘组织中Bax表达降低(P<0.01),Bcl-2表达升高(P<0.001),Caspase-3活性降低(P<0.01)。结论:胶艾汤能够上调miR-29α-3p的表达,调控线粒体凋亡,干预滋养层细Objective:To explore the mechanism of mitochondrial regulation by Jiao Ai decoction in a model of idiopathic miscarriage.Methods:Cell experiments and animal experiments were conducted.In the cell experiment,the control group,the Jiao Ai decoction group,the miR-29α-3p mimic group,and the Jiao Ai decoction+miR-29α-3p mimic group were set up.Human chorionic trophoblast cells HTR-8/SVneo were cultured and treated,and the expression of miR-29α-3p,mitochondrial apoptosis,cytochrome C release,apoptosis-related protein expression,cell migration and invasion ability were detected.In the animal experiment,pregnant C57BL/6J mice were selected to establish a miscarriage model,and the groups were treated with Jiao Ai decoction by gavage and miR-29α-3p mimic by intraperitoneal injection.The miscarriage situation,placental histology changes,miR-29α-3p expression,mitochondrial apoptosis-related indicators,and oxidative stress indicators were observed.Results:In the cell experiment,compared with the control group,the expression level of miR-29α-3p in the Jiao Ai decoction group was significantly increased at 48 hours(P<0.001),the expression level in the miR-29α-3p mimic group was also significantly higher than that in the control group,and the expression level in the Jiao Ai decoction+miR-29α-3p mimic group was the highest.Jiao Ai decoction and miR-29α-3p mimic could reduce the change of mitochondrial membrane potential,reduce the release of cytochrome C,regulate the expression of apoptosis-related proteins(Bax expression decreased,Bcl-2 expression increased,Caspase-3 activity decreased),and make them shift to the anti-apoptotic direction,thereby inhibiting mitochondrial apoptosis,and the combined use had a better effect.Jiao Ai decoction and miR-29α-3p mimic could also promote the migration and invasion ability of cells,and the combined use had a more significant effect.In the animal experiment,the miscarriage rate in the miscarriage model group was higher,at(31.17±0.67)%.The miscarriage rate in the Jiao Ai decoction

关 键 词：自然流产 胶艾汤 miR-29α-3p 线粒体 凋亡 滋养层细胞 

分 类 号：R714.21[医药卫生—妇产科学]