不同抗体分型Hp感染对慢性萎缩性胃炎血清胃功能的影响  

Effects of Different Antibody Types of Hp Infection on the Serum Gastric Function in Chronic Atrophic Gastritis

作　　者：王艳红 钟洁 张里 单保东 姚曼曼 李胜男 曹希祥 李莉 费素娟 庞训雷 WANG Yanhong;ZHONG Jie;ZHANG Li(Department of Gastroenterology,Affiliated Hospital of Xuzhou Medical University,Jiangsu 221004,China)

机构地区：[1]徐州医科大学附属医院消化内科,221004 [2]徐州医科大学研究生学院,221006

出　　处：《医学研究杂志》2024年第11期105-112,共8页Journal of Medical Research

基　　金：江苏省重点实验室开放课题(XZSYSKF2021029);江苏省徐州市科技发展重点项目(社会发展)(KC22233);徐州医科大学附属医院院课题(2020KA003,2023ZL19)。

摘　　要：目的 检测慢性萎缩性胃炎(chronic atrophic gastritis, CAG)患者血清胃功能的表达并对其Hp进行抗体分型检测,以期了解不同抗体分型组合的Hp感染对CAG血清胃功能的影响。方法 选取徐州医科大学附属医院门诊的患者进行胃部相关的检查后分为慢性非萎缩性胃炎(chronic non-atrophic gastritis, CNAG)组和CAG组,荧光免疫层析法检测血清胃功能表达水平,Western blot法检测Hp抗体分型。分析不同抗体分型组合的Hp感染对CAG血清胃功能的影响。结果 与CNAG组比较,CAG组患者G17水平明显增高;CAG组患者Ure阳性组、Ure阳性+CagA阳性和Ure阳性+CagA阳性+VacA阳性比例明显增高(P=0.017);Hp阴性组中,CAG组G17的表达水平明显高于CNAG组(P=0.024);Ure阳性+CagA阳性组中,CAG组胃蛋白酶原ⅡPGⅡ的表达水平明显低于CNAG组(P=0.019);在CAG组中,Ure阳性+VacA阳性和Ure阳性+CagA阳性+VacA阳性组胃蛋白酶原Ⅰ(PGⅠ)/PGⅡ比值(PGR)明显低于Ure阳性+CagA阳性组(P<0.05);CAG组患者中,中重度萎缩组PGⅠ的表达水平显著低于轻度萎缩组(P<0.001),而中重度萎缩组PGR也显著低于轻度萎缩组(P<0.001);中重度萎缩组患者Ure阳性+CagA阳性、Ure阳性+VacA阳性和Ure阳性+CagA阳性+VacA阳性的比例均明显高于轻度萎缩组(P=0.013)。结论CAG组患者G17水平明显增高,不同抗体分型的Hp感染影响了CAG的发生和萎缩的严重程度。VacA阳性的Hp感染(合并或不合并CagA阳性)均可导致CAG患者存在较低的PGR,而单独CagA阳性的Hp感染导致CAG患者存在较高的PGR。Objective To detect the expression of serum gastric function in patients with chronic atrophic gastritis(CAG)and determine the effect of Hp infection with different antibody types on serum gastric function in CAG.Methods The patients in outpatient department of Affiliated Hospital of Xuzhou Medical University were selected for gastric examination and divided into two groups:chronic non-atrophic gastritis(CNAG)group and CAG group.The serum levels of gastric function expression were detected using fluorescence immunochromatography,and the Hp antibody type was determined by Western blot,to analyze the effects of different Hp antibody types on the gastric function of CAG serum.Results Compared with the CNAG group,the G17 level in the CAG group was significantly increased.The proportion of Ure-positive individuals,Ure-positive+CagA-positive individuals,and Ure-positive+CagA-positive+VacA-positive individuals in the CAG group showed a significant increase(P=0.017).In the Hp-negative group,the expression level of G17 in the CAG group was higher than that in the CNAG group(P=0.024),and the expression level of PGⅡin Ure-positive+CagA-positive individuals was lower than that in C NAG group(P=0.019).In the CAG group,the PGR(PGⅠ/PGⅡ)of Ure-positive+VacA-positive and Ure-positive+CagA-positive+VacA-positive individuals was lower than that of Ure-positive+CagA-positive individuals(P<0.05).In addition,PGⅠlevels were lower in moderate and severe atrophy groups compared to mild atrophy groups within the CAG group(P<0.001),and PGR levels were also lower in moderate and severe atrophy groups compared to mild atrophy groups(P<0.001).Furthermore,proportions of Ure-positive+CagA-positive,Ure-positive+VacA-positive,and Ure-positive+CagA-positive+VacA-positive individuals were higher in moderate and severe atrophy groups compared to mild atrophy groups(P=0.013).Conclusion The level of G17 was increased in the CAG group,and Hp infection with different types of antibodies affected the occurrence and severity of CAG atrophy.VacA-po

关 键 词：抗体分型 HP 慢性萎缩性胃炎 血清胃功能 

分 类 号：R446[医药卫生—诊断学]