岗梅清咽合剂HPLC特征图谱及同时测定多成分含量  

HPLC characteristic chromatogram of Gangmei Qingyan Mixture and simultaneous determination of multiple components

作　　者：林秀娟 刘翠珍 雷晓椿 曾德浩[2] 丘振文[1,2,3] 李得堂 LIN Xiujuan;LIU Cuizhen;LEI Xiaochun;ZENG Dehao;QIU Zhenwen;LI Detang(College I of Clinical Medicine,Guangzhou University of Chinese Medicine,Guangdong Guangzhou 510405,China;Department of Pharmacy,First Affiliated Hospital,Guangzhou University of Chinese Medicine,Guangdong Guangzhou 510405,China;Guangdong Clinical Research Academy of Chinese Medicine,Guangdong Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一临床医学院,广东广州510405 [2]广州中医药大学第一附属医院药学部,广东广州510405 [3]广东省中医临床研究院,广东广州510405

出　　处：《中国医院药学杂志》2024年第23期2700-2706,共7页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金面上项目(编号:82374526);广州市科技计划项目市校(院)联合资助项目(编号:2023A03J0299);广州市科技计划项目(编号:202201020457);全国名老中医药专家传承工作室建设项目(编号:国中医药人教函[2022]75号)。

摘　　要：目的:建立岗梅清咽合剂的高效液相色谱(HPLC)特征图谱,以及同时测定多个特征性成分含量。方法:采用HPLC法建立10批岗梅清咽合剂的特征图谱,评价其相似度,确定共有特征峰,并对各共有峰进行归属;以特征图谱共有峰峰面积为分析对象,进行化学计量法分析,根据变量重要性投影(VIP)值大于1,筛选出差异性成分,并对6个特征性化学成分同时测定其含量。结果:10批岗梅清咽合剂共标定出21个共有特征峰,10批样品的相似度均大于0.950,与对照品比对出了11个特征峰。CA和PCA结果显示,10批样品可聚为3类;OPLS-DA结果显示,VIP值大于1的成分筛选出了10个成分,依次为4(没食子酸),10、9(咖啡酸),13、8(隐绿原酸),2、3、17(异绿原酸C),15(异绿原酸A),7(绿原酸)号峰。10批样品中没食子酸、绿原酸、隐绿原酸、咖啡酸、异绿原酸A、异绿原酸C的含量分别为0.097~0.591、0.055~0.131、0.051~0.200、0.402~1.171、0.013~0.087、0.013~0.123 mg·mL^(-1)。结论:建立的HPLC特征图谱方法稳定可行,可用于岗梅清咽合剂的质量控制。OBJECTIVE To establish the characteristic chromatogram of Gangmei Qingyan Mixture(GQM)with high performance liquid chromatography(HPLC)and simultaneous content determination of multiple characteristic components.METHODS HPLC was utilized for establishing the characteristic atlas of 10 batches of GQM,evaluating their similarity,determining the common characteristic peaks and assigning the common peaks.Stoichiometric analysis was performed through taking the common peak area of characteristic map as a variable.The differential components were screened out according to the importance projection(VIP)value of variable>1.And the contents of 6 characteristic chemical components were determined simultaneously.RESULTS A total of 21 common characteristic peaks were identified in 10 batches of GQM.The similarity of 10 preparation batches was>0.950 and 11 characteristic peaks were detected when compared with control products.The results of cluster analysis(CA)and principal component analysis(PCA)revealed that 10 batches of samples could be classified into three distinct categories.The results of orthogonal partial least squares discriminant analysis(OPLS-DA)indicated that 10 components were screened out for components with VIP value>1,namely 4(gallic acid),10/9(caffeic acid),13/8(cryptochlorogenic acid),2/3/17(isochlorogenic acid C),15(isochlorogenic acid A)and 7(chlorogenic acid).The contents of gallic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,isochlorogenic acid A and isochlorogenic acid C in 10 batches of samples were(0.097-0.591),(0.055-0.131),(0.051-0.20),(0.402-1.171),(0.019-0.104)and(0.013-0.123)mg·mL^(-1).CONCLUSION Both stable and feasible,HPLC characteristic mapping may be employed for quality control of GQM.

关 键 词：岗梅清咽合剂 特征图谱 化学计量法 含量测定 

分 类 号：R917[医药卫生—药物分析学]