血清lncRNA NNT-AS1、miR-582-5p与支气管哮喘患儿气道炎症、气道高反应的关系研究  

Research on the Relationship between Serum lncRNA NNT-AS1 and miR-582-5p and Airway Inflammation and Airway Hyperresponsiveness in Children with Bronchial Asthma

作　　者：张珍 刘光辉 梁慧玲 吴开松[2] 张珒珒 董翔 ZHANG Zhen;LIU Guang-hui;LIANG Hui-ling;WU Kai-song;ZHANG Jin-jin;DONG Xiang(Department of Allergic Reaction,Wuhan University Zhongnan Hospital,Wuhan,Hubei,430071,China;Department of Respiratory and Critical Care Medicine,Wuhan University Zhongnan Hospital,Wuhan,Hubei,430071,China)

机构地区：[1]武汉大学中南医院过敏反应科,湖北武汉430071 [2]武汉大学中南医院呼吸与危重症医学科,湖北武汉430071

出　　处：《现代生物医学进展》2024年第22期4256-4258,共3页Progress in Modern Biomedicine

基　　金：湖北省自然科学基金面上项目(2021CFB308)。

摘　　要：目的:探讨血清长链非编码核糖核酸(lncRNA)烟酰胺核苷酸反义转氢酶RNA1(NNT-AS1)、微小核糖核酸(mi R)-582-5p与支气管哮喘患儿气道炎症、气道高反应的关系。方法:选择120例支气管哮喘患儿,根据相关指南分为缓解期组(n=52)与发作期组(n=68)。另选择同期60例健康儿童作为对照组。对比三组血清lncRNA NNT-AS1、mi R-582-5p相对表达量及气道炎症细胞因子[白介素-17(IL-17)、肿瘤坏死因子-α(TNF-α)]水平、气道反应性指标[基础呼吸阻力(Rrs cont)、最小诱发累积剂量(Dmin)、基础呼吸传导率(Grs cont)]。Pearson相关性检验分析血清lncRNA NNT-AS1、mi R-582-5p表达与血清气道炎症细胞因子、气道反应性指标的关系。结果:缓解期组、发作期组lncRNA NNT-AS1相对表达量、血清IL-17、TNF-α水平、Rrs cont高于对照组,且发作期组高于缓解期组(P<0.05)。缓解期组、发作期组mi R-582-5p相对表达量、Grs cont、Dmin低于对照组,且发作期组低于缓解期组(P<0.05)。支气管哮喘患儿血清lncRNA NNT-AS1表达与IL-7、TNF-α、Rrs cont呈正相关(r>0,P<0.05),与Grs cont、Dmin呈负相关(r<0,P<0.05);血清mi R-582-5p表达与IL-17、TNF-α、Rrs cont呈负相关(r<0,P<0.05),与Grs cont、Dmin呈正相关(r>0,P<0.05)。结论:支气管哮喘患儿血清lncRNA NNT-AS1呈高表达,mi R-582-5p呈低表达,二者表达与患儿哮喘分期、气道炎症、气道高反应密切相关。Objective:To investigate the relationship between serum long non-coding RNA(lncRNA)nicotinamide nucleotide antisense transferase RNA1(NNT-AS1),microRNA(miR)-582-5p and airway inflammation and airway hyperresponsiveness in children with bronchial asthma.Methods:120 children with bronchial asthma were divided into remission group(n=52)and attack group(n=68)according to the relevant guidelines.Another 60 healthy children in the same period were selected as the control group.The relative expression of serum lncRNA NNT-AS1,miR-582-5p and serum airway inflammatory cytokines[interleukin-17(IL-17),tumor necrosis factor-α(TNF-α)],airway responsiveness indicators[basic respiratory resistance(Rrs cont),minimum induced cumulative dose(Dmin),basic respiratory conductivity(Grs cont)]were compared among three groups.The relationship between serum lncRNA NNT-AS1,miR-582-5p expression and airway inflammatory cytokines,airway responsiveness indicators were analyzed by Pearson correlation test.Results:The relative expression of lncRNA NNT-AS1,serum levels of IL-17,TNF-αand Rrs cont in remission group and attack group were higher than those in control group,and those in attack group were higher than those in remission group(P<0.05).The relative expression of miR-582-5p,Grs cont and Dmin in remission group and attack group were lower than those in control group,and those in attack group were lower than those in remission group(P<0.05).The expression of serum lncRNA NNT-AS1 in bronchial asthma children was positively correlated with IL-7,TNF-αand Rrs cont(r>0,P<0.05),and negatively correlated with Grs cont and Dmin(r<0,P<0.05).The expression of serum miR-582-5p was negatively correlated with IL-17,TNF-αand Rrs cont(r<0,P<0.05),and positively correlated with Grs cont and Dmin(r>0,P<0.05).Conclusion:Serum lncRNA NNT-AS1 is highly expressed and miR-582-5p is lowly expressed in children with bronchial asthma,the expression of both are closely related to asthma stage,airway inflammation and airway hyperresponsiveness.

关 键 词：lncRNA NNT-AS1 mi R-582-5p 支气管哮喘 儿童 气道炎症 气道高反应 

分 类 号：R725.6[医药卫生—儿科]