肥胖型多囊卵巢综合征患者外周血淋巴细胞miR-27a、miR-141与PI3K/AKT信号通路的相关性分析  

Correlation Analysis of miR-27a,miR-141 and PI3K/AKT Signaling Pathway in Peripheral Blood Lymphocytes of Obese Patients with Polycystic Ovary Syndrome

作　　者：孙潇潇 成佳景 张君宜[3] 陆思芸 陈宇航 李武 SUN Xiao-xiao;CHENG Jia-jing;ZHANG Jun-yi;LU Si-yun;CHEN Yu-hang;LI Wu(Clinical Medicine School of Shanghai Tenth Hospital of Nanjing Medical University,Shanghai,211103,China;Department of Gynaecology and Obstetrics,Tenth People's Hospital of Tongji University,Shanghai,200040,China;Department of Endocrinology,Tenth People's Hospital of Tongji University,Shanghai,200040,China)

机构地区：[1]南京医科大学上海十院临床医学院,上海211103 [2]同济大学附属第十人民医院妇产科,上海200040 [3]同济大学附属第十人民医院内分泌科,上海200040

出　　处：《现代生物医学进展》2024年第22期4291-4293,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年项目(81502451)。

摘　　要：目的:探讨肥胖型多囊卵巢综合征(PCOS)患者外周血淋巴细胞微小核糖核酸(MicroRNA,miR)-27a、miR-141与磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)信号通路的相关性。方法:选取110例PCOS患者,根据体质量指数(BMI)分为非肥胖组(40例)和肥胖组(70例)。检测外周血淋巴细胞miR-27a、miR-141和外周血PI3K mRNA、AKT mRNA表达水平,采用Pearson法分析相关性。结果:对比非肥胖组,肥胖组PCOS患者外周血淋巴细胞miR-27a、miR-141的表达水平更高,但外周血PI3K mRNA、AKT mRNA的表达水平更低(P<0.05)。PCOS患者外周血淋巴细胞miR-27a、miR-141表达与外周血PI3K mRNA、AKT mRNA表达呈负相关(P<0.05)。结论:肥胖型PCOS患者外周血淋巴细胞miR-27a、miR-141表达增高,外周血PI3K mRNA、AKT mRNA表达降低。miR-27a、miR-141高表达和与PCOS患者PI3K/Akt信号通路受到抑制有关。Objective:To investigate the correlation between microRNA(miR)-27a,miR-141 and phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)signa/ing pathway in peripheral blood lymphocytes of obese patients with polycystic ovary syndrome(PCOS).Methods:110 PCOS patients were selected,and patients were divided into non-obese group(40 cases)and obese group(70 cases)according to body mass index(BMI).The levels of miR-27a,miR-141 expression in peripheral blood lymphocytes and PI3K mRNA,AKT mRNA in peripheral blood were detected,and the correlation were analyzed by Pearson method.Results:Compared with non-obese group,the levels of miR-27a and miR-141 expression in peripheral blood lymphocytes of PCOS patients in obese group were higher,the expression levels of PI3K mRNA and AKT mRNA in peripheral blood were lower(P<0.05).The levels of miR-27a and miR-141 expression in peripheral blood lymphocytes of PCOS patients was negatively correlated with the expression of PI3K mRNA and AKT mRNA in peripheral blood(P<0.05).Conclusion:The levels of miR-27a and miR-141 expression in peripheral blood lymphocytes of obese PCOS patients increased,and the levels of PI3K mRNA and AKT mRNA expression in peripheral blood decreased.The high expression of miR-27a and miR-141 are related to the inhibition of PI3K/Akt signaling pathway in PCOS patients.

关 键 词：肥胖型多囊卵巢综合征 miR-27a miR-141 PI3K/AKT信号通路 

分 类 号：R711.7[医药卫生—妇产科学]