纳米银涂层可吸收缝线对小鼠皮肤切口缝合线道的远期作用  

Long term efficacy of absorbable sutures coated with silver nanoparticles on the suture track of skin incisions in mice

作　　者：何峰[1] 杜娟[2] 宋岩彪 叶茂 魏延栋[5] 刘雪来[4] He Feng;Du Juan;Song Yanbiao;Ye Mao;Wei Yandong;Liu Xuelai(Department of Biochemistry and Immunology,Capital Institute of Pediatrics,Beijing 100020,China;Emergency,Critical and Translational Medicine Laboratory,Jilin Province People's Hospital,Changchun 130021,China;Central Laboratory,the Second Hospital of Hebei Medical University,Shijiazhuang 050061,China;Department of General Surgery,Capital Institute of Pediatrics Affiliated Children Hospital,Beijing 100020,China;Department of Neonatal Surgery,Capital Institute of Pediatrics Affiliated Children Hospital,Beijing 100020,China)

机构地区：[1]首都儿科研究所生化免疫学研究室,北京100020 [2]吉林省人民医院急危重症科研与转化实验室,长春130021 [3]河北医科大学第二医院中心实验室,石家庄050061 [4]首都儿科研究所附属儿童医院普通外科,北京100020 [5]首都儿科研究所附属儿童医院新生儿外科,北京100020

出　　处：《中华烧伤与创面修复杂志》2024年第12期1184-1192,共9页Chinese Journal of Burns And Wounds

基　　金：北京市自然科学基金面上项目(7222015);2024-2025北京市卫生健康委“一带一路”卫生健康国际合作项目和世界卫生组织合作中心项目;北京市中医药科技发展资金项目(JJ-2020-50);首都儿科研究所所级课题(LCYJ-2023-07)。

摘　　要：目的探讨纳米银涂层可吸收缝线对小鼠皮肤切口缝合线道的远期作用。方法该研究为实验研究。将18只10~12周龄雌雄各半BALB/c小鼠,按照随机数字表法分为普通薇乔线组、抗生素薇乔线组和纳米银薇乔线组,每组6只,雌雄各半。于所有小鼠背部制备皮肤切口后,对普通薇乔线组、抗生素薇乔线组和纳米银薇乔线组小鼠皮肤切口分别采用可吸收薇乔线、三氯生抗菌涂层可吸收薇乔线和纳米银涂层可吸收薇乔线行皮内连续缝合。术后25 d,采集切口缝合线道皮肤组织,行免疫组织化学染色观测小鼠切口缝合线道F4/80、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)阳性面积百分比,行原位末端标记(TUNEL)染色观测小鼠切口缝合线道TUNEL染色阳性面积百分比,行Masson染色观察小鼠切口缝合线道胶原纤维形成情况,行免疫组织化学染色观测小鼠切口缝合线道组织蛋白酶K阳性面积百分比。以上实验中样本数均为6。结果术后25 d,纳米银薇乔线组小鼠切口缝合线道F4/80、IL-6及TNF-α阳性面积百分比[(19.2±1.6)%、(20.2±1.7)%、(16.0±1.6)%]均明显低于普通薇乔线组[(100±6.4)%、(100±7.6)%、(100±9.6)%]和抗生素薇乔线组[(47.2±3.2)%、(53.8±5.0)%、43.2%,P<0.05],抗生素薇乔线组小鼠切口缝合线道F4/80、IL-6及TNF-α阳性面积百分比均明显低于普通薇乔线组(P<0.05)。术后25 d,纳米银薇乔线组小鼠切口缝合线道TUNEL染色阳性面积百分比明显低于普通薇乔线组和抗生素薇乔线组(P值均<0.05),抗生素薇乔线组小鼠切口缝合线道TUNEL染色阳性面积百分比明显高于普通薇乔线组(P<0.05)。术后25 d,普通薇乔线组小鼠切口缝合线道胶原纤维沉积不明显,抗生素薇乔线组小鼠切口缝合线道无明显胶原纤维,纳米银薇乔线组小鼠切口缝合线道胶原纤维致密程度最高且排列有序。术后25 d,纳米银薇乔线组小鼠切口缝合线道组织蛋白�Objective To explore the long term efficacy of absorbable sutures coated with silver nanoparticles on the suture track of skin incisions in mice.Methods This study was an experimental research.Eighteen half male and half female BALB/c mice aged 10-12 weeks were divided into normal Vicryl suture group,antibiotic Vicryl suture group,and silver nanoparticle Vicryl suture group according to the random number table,with 6 mice in each group,half male and half female.After the skin incisions were created on the back of all mice,the skin incisions of mice in normal Vicryl suture group,antibiotic Vicryl suture group,and silver nanoparticle Vicryl suture group were performed with continuous intradermal suture by absorbable Vicryl sutures,absorbable Vicryl sutures coated with triclosan antimicrobial,and absorbable Vicryl sutures coated with silver nanoparticles,respectively.On the 25 th day after surgery,the skin tissue in the suture track of incisions was collected,immunohistochemical staining was used to detect the percentages of F4/80,interleukin-6(IL-6),and tumor necrosis factorα(TNF-α)positive areas in the suture track of incisions of mice,the TdT-mediated dUTP nick-end labeling(TUNEL)staining was used to detect the percentage of TUNEL staining positive area in the suture track of incisions of mice,Masson staining was used to observe the collagen fiber formation in the suture track of incisions of mice,and immunohistochemical staining was used to detect the percentage of tissue cathepsin K positive area in the suture track of incisions of mice.The number of the above experimental samples was 6.Results On the 25^(th) day after surgery,the percentages of F4/80,IL-6,and TNF-αpositive areas in the suture track of incisions of mice in silver nanoparticle Vicryl suture group((19.2±1.6)%,(20.2±1.7)%,and(16.0±1.6)%)were significantly lower than those in normal Vicryl suture group((100±6.4)%,(100±7.6)%,and(100±9.6)%)and antibiotic Vicryl suture group((47.2±3.2)%,(53.8±5.0)%,and 43.2%,P<0.05).The percentages of F4/80

关 键 词：创伤和损伤 炎症 细胞凋亡 纳米颗粒 创面修复 

分 类 号：R318.08[医药卫生—生物医学工程]