兔病毒性出血症卵黄抗体对育肥兔抗体效价变化研究  

Study on Changes in Antibody Titer of Rabbit Hemorrhagic Disease Egg Yolk Antibodyin Fattening Rabbits

作　　者：任永军[1,2] 袁定胜 杨泽晓[3] 邝良德 郭志强[1,2] REN Yongjun;YUAN Dingsheng;YANG Zexiao;KUANG Liangde;GUO Zhiqiang(Sichuan Animal Science Academy,chengdu 610066;Animal Breeding and Genetics key Laboratory of Sichuan Province,chengdu 610066;College of Veterinary Medicine,Sichuan Agricultural University,chengdu 611303)

机构地区：[1]四川省畜牧科学研究院,成都610066 [2]动物遗传育种四川省重点实验室,成都610066 [3]四川农业大学动物医学院,成都611303

出　　处：《中国养兔》2024年第5期13-15,共3页Chinese Journal of Rabbit Farming

基　　金：兔病毒性出血症二价口服疫苗技术成果引进与示范推广(2023ZHYZ0006);油樟油绿色饲料产品开发关键技术研究与示范(2023YFN0076);十四五育种攻关项目:优质肉兔配套系选育(2021YFYZ0033);财政专项:肉兔配套系培育及地方遗传资源的保护利用研究(SASA2023CZYX004)。

摘　　要：选用无RHDV、RHDV2母源抗体35日龄断奶仔兔120只,分为4组,每组30只,分别为对照组与试验1、2、3组,试验1、2、3组每千克饲粮中分别添加兔病毒病出血症卵黄抗体0.4、0.6、0.8 g,对照组空白对照,每组设5个重复,每个重复6只(公母各半),在试验开始后第7、14、28、42天,分别耳缘静脉采集各组试验兔血液并分离血清,并检测RHDV、RHDV2抗体水平。试验结果显示:对照组在第7、14、28、42天抗RHDV、RHDV2抗体水平均为0;试验1组抗RHDV抗体水平分别为1∶50、1∶200、1∶500、1∶1000,抗RHDV2抗体水平分别为1:50、1∶100、1∶500、1∶1000;试验2组抗RHDV抗体水平分别为1∶100、1∶300、1∶1000、1∶2000,抗RHDV2抗体水平分别为1∶50、1∶200、1∶1000、1∶1000;试验3组抗RHDV抗体水平分别为1∶200、1∶500、1∶2000、1∶2000,抗RHDV2抗体水平分别为1∶200、1∶500、1∶2000、1∶2000。说明当每千克饲粮中添加兔病毒性出血症卵黄抗体添加量为0.8 g时,育肥兔在14 d就可获得较高的RHDV、RHDV2抗体水平。In this study,12035 day old weaned rabbits without RHDV and RHDV2 maternal antibodies were selected and divided into 4 groups,with 30 rabbits in each group,namely the control group and Experiment 1,2,and 3 groups.Experiment 1,2,and 3 groups were added 0.4g,0.6g,and 0.8g of rabbit hemorrhagic disease egg yolk antibody(Ig Y)per kilogram of diet,respectively.The control group was blank control,with 5 replicates in each group,6 rabbits in each replicate(half male and half female).At the 7th,14th,28th,and 42nd day after the experiment,blood of rabbits in each group were collected through ear vein,and the serum was separated.RHDV and RHDV2 antibody levels were measured.The experimental results showed that the levels of anti RHDV and RHDV2 antibodies in the control group were 0,the levels of anti RHDV antibodies in Experiment 1 group were 1∶50,1∶200,1∶500,and 1:1000,respectively,and the levels of anti RHDV2 antibodies were 1∶50,1∶100,1∶500,and 1∶1000;the levels of anti RHDV antibodies in experimental 2 group were 1∶100,1∶300,1∶1000,and 1∶2000,respectively,and the levels of anti RHDV2 antibodies were 1∶50,1∶200,1∶1000,and 1∶1000;the levels of anti RHDV antibodies in the experimental 3 group were 1∶200,1∶500,1∶2000,and 1∶2000,respectively,and the levels of anti RHDV2 antibodies were 1∶200,1∶500,1∶2000,and 1∶2000.The results showed that when 0.8 g of rabbit hemorrhagic disease egg yolk antibody was added to each kilogram of diet,the fattening rabbits could obtain higher levels of RHDV and RHDV2 antibodies at 14 days.

关 键 词：RHDV RHDV2 卵黄抗体 抗体效价 

分 类 号：S829.1[农业科学—畜牧学]