羽毛针禾(Stipagrostis pennata)阿拉伯呋喃糖苷酶基因SpARAF1的克隆与表达  

Cloning and expression of Alpha-L-Arabinofuranosidases Gene SpARAF1 from Stipagrostis pennata

作　　者：尹珊 唐蕊 尹清乐 王斐[1,2,3] 李榕[1,2,3] 李鸿彬 Shan Yin;Rui Tang;Qingle Yin;Fei Wang;Rong Li;Hongbin Li(College of Life Science,Shihezi University,Shihezi 832003,Xinjiang,China;Xinjiang Production and Construction Corps Key Laboratory of Oasis Town and Mountain-basin System Ecology,Shihezi University,Shihezi 832003,Xinjiang,China;Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education,Shihezi University,Shihezi 832003,Xinjiang,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]石河子大学绿洲城镇与山盆系统生态兵团重点实验室,新疆石河子832003 [3]石河子大学新疆植物药资源利用教育部重点实验室,新疆石河子832003

出　　处：《中国沙漠》2024年第6期48-57,共10页Journal of Desert Research

基　　金：国家自然科学基金项目(32060082);兵团自然科学支持计划项目(2024DA061);石河子大学高层次人才科研启动项目(校20220078);石河子大学科技计划项目(KX008505);兵团科技计划项目(2020BC002);促进与加拿大、澳大利亚、新西兰及拉美地区科研合作与高层次人才培养项目。

摘　　要：L-阿拉伯呋喃糖苷酶(Alpha-L-Arabinofuranosidase ARAF)在植物细胞壁形成和逆境胁迫响应中发挥着重要作用,为了探究该基因参与羽毛针禾(Stipagrostis pennata)抵御非生物胁迫和影响其根部沙套发育的机制及功能,本研究以沙漠植物羽毛针禾为对象,克隆得到SpARAF1基因并对其进行生物信息学、亚细胞定位和实时荧光定量聚合酶链式反应(PCR)分析。结果表明:羽毛针禾SpARAF1基因编码区序列全长为1 173 bp,编码391个氨基酸的亲水性无跨膜结构的蛋白,相对分子质量为43.3 kD。该基因编码的蛋白属于Alpha-L-AF-C超家族,与水稻和玉米亲缘关系较近。亚细胞定位显示SpARAF1定位在细胞壁。qRT-PCR结果显示SpARAF1基因的表达显著受到盐、干旱和高温等非生物胁迫的诱导,表明该基因参与羽毛针禾对非生物胁迫的响应过程。蛋白质互作预测分析显示SpARAF1与碳水化合物激酶和聚半乳糖醛酸酶存在可能的相互作用。Alpha-L-Arabinofuranosidase(ARAF)plays an important role in plant cell wall formation and response to stress.In order to explore the mechanism and function of gene involved in resistance to abiotic stress and influence on the development of root sand jacket in grass.In this study,SpARAF1 gene was cloned and analyzed by bioinformatics,subcellular localization and real-time fluorescence quantitative PCR.The total length of SpARAF1 coding region is 1173 bp,encoding 391 amino acids hydrophilic non-transmembrane protein,the relative molecular weight is 43.3 kD.Phylogenetic analysis showed that the protein encoded by this gene belongs to the Alpha-L-AF-C superfamily and is closely related to rice and maize.Subcellular localization showed that SpARAF1 was localized in the cell wall.The results of qRT-PCR showed that the expression of SpARAF1 was significantly induced by abiotic stress such as salt,drought and high temperature,which indicated that SpARAF1 was involved in the response process of Stipagrostis pennata to abiotic stress.Protein interaction prediction analysis showed that SpARAF1 may interact with carbohydrate kinase and polygalacturonase.

关 键 词：羽毛针禾(Stipagrostis pennata) SpARAF1 阿拉伯呋喃糖苷酶 非生物胁迫 亚细胞定位 

分 类 号：Q943[生物学—植物学]