Enterobacter sp.菌株的耐锰特性及表征  

Manganese resistance and characterization of Enterobacter sp.strain

作　　者：刘少芳 占梦婷 黄雨珊 陈敏贤 蔡霓 胡志宏 江雪 Shaofang Liu;Mengting Zhan;Yushan Huang;Minxian Chen;Ni Cai;Zhihong Hu;Xue Jiang(Laboratory of Natural Microbial Medicine Research of Jiangxi Province/Key Laboratory of Microbial resources and metabolism of Nanchang City,Jiangxi Science and Technology Normal University,Nanchang 330013,China;Fundamental Science on Radioactive Geology and Exploration Technology Laboratory,East China University of Technology,Nanchang 330013,China;Hubei Biopesticide Engineering Research Centre,Wuhan 430064,China)

机构地区：[1]江西科技师范大学天然微生物药物研究江西省重点实验室/南昌市微生物资源与代谢重点实验室,江西南昌330013 [2]东华理工大学放射性地质与勘探技术国防重点学科实验室,江西南昌330013 [3]湖北省生物农药工程研究中心,湖北武汉430064

出　　处：《中国沙漠》2024年第6期299-306,共8页Journal of Desert Research

基　　金：国家自然科学基金项目(32360080);江西省自然科学基金项目(20242BAB20286);放射性地质与勘探技术国防重点学科实验室开放基金项目(2022RGET06)。

摘　　要：通过富集培养的方法,从某铀矿周边的稻田土壤中筛选出了一株表现出较强耐锰能力的细菌菌株Enterobacter sp.,命名为NM3。经过菌落形态观察、革兰氏染色以及16S rDNA序列分析的鉴定,确认该细菌归于Enterobacter属。为了研究NM3菌株吸附Mn2+的效率及相关吸附机理,运用了原子吸收光谱、扫描电镜和傅里叶红外光谱等多种仪器进行深入分析。结果表明:该菌株对Mn2+的耐受性高达8000 mg·L^(-1)。在Mn^(2+)浓度为4000mg·L^(-1)条件下经过60 h反应,去除率可达93.3%。参与这一吸附过程的主要官能团包括羟基、烷基和酰胺基团等。进一步检测NM3菌株的促生特性及其对青菜富集Mn^(2+)的影响时发现,该菌株具备产生生长素IAA的能力,能够显著提升锰胁迫下青菜生物量34.13%~56.09%,并且使青菜中的Mn2+含量下降50.09%。A bacterial strain exhibiting strong manganese(Mn)tolerance was isolated from paddy soil surrounding a uranium mine using the enrichment culture method and designated as NM3.The bacterium was identified as belonging to the Enterobacter genus through colony morphology,Gram staining,and 16S rDNA sequence analysis.To investigate the adsorption efficiency of Mn^(2+)by the NM3 strain and the associated adsorption mechanisms,we employed atomic absorption spectroscopy,scanning electron microscopy,and Fourier-transform infrared spectroscopy for comprehensive analysis.The results indicated that the strain tolerated Mn^(2+)concentrations of up to 8000 mg·L^(-1).After a 60-hour reaction period at a Mn^(2+)concentration of 4000 mg·L^(-1),the removal rate reached 93.3%.The primary functional groups involved in this adsorption process were identified as hydroxyl,alkyl,and amide groups.Further evaluation of the growth-promoting properties of the NM3 strain and its impact on Mn^(2+)accumulation in green vegetables revealed that the strain produced auxin indole-3-acetic acid(IAA),which significantly enhanced the biomass of green vegetables under Mn stress,with increases ranging from 34.13%to 56.09%.Additionally,the Mn^(2+)content in the green vegetables decreased by 50.09%.In summary,the NM3 strain not only demonstrates high tolerance and strong adsorption capacity but also represents a promising new microbial resource for mitigating soil Mn pollution,thereby ensuring vegetable safety.

关 键 词：耐锰细菌 去除率 植物促生 锰吸附 

分 类 号：Q935[生物学—微生物学]