1株不完全耐受鼠疫诊断噬菌体的田鼠型鼠疫耶尔森菌表型特征  

The phenotypic characteristics of a Microtus strain of Yersinia pestis from Lasiopodomys brandtii with partial resistance to plague diagnostic phage

作　　者：李存香[1] 祁芝珍[1] 杨晓艳[1] 李翔[1] 吴海生[1] 张丽 张晓璐 赵海红[1] 靳娟[1] 辛有全[1] LI Cun-xiang;QI Zhi-zhen;YANG Xiao-yan;LI Xiang;WU Hai-sheng;ZHANG Li;ZHANG Xiao-lu;ZHAO Hai-hong;JIN Juan;XIN You-quan(Key Laboratory for Plague Prevention and Control of Qinghai Province,Qinghai Institute for Endemic Diseases Prevention and Control,Xining,Qinghai 810021,China)

机构地区：[1]青海省地方病预防控制所鼠疫菌专业实验室,青海西宁811602

出　　处：《中国媒介生物学及控制杂志》2024年第6期644-647,共4页Chinese Journal of Vector Biology and Control

基　　金：青海省医药卫生科技项目重点课题(2022-wjzd-06);青海省地方病预防控制所/国家卫生健康委鼠疫防治研究重点实验室(共建)。

摘　　要：目的分析田鼠型鼠疫耶尔森菌(鼠疫菌)90017表型特征,为探索细菌抵抗噬菌体机制、鼠疫疫源地鼠疫菌长期保存机制和传染病进化流行病学研究提供理论依据。方法分别用2×10^(9)、1×10^(6)、1×10^(5)个/ml浓度田鼠型鼠疫菌株悬液皮下感染BALB/c小鼠,动物死亡后脏器经压印培养,观察动物实验前、后培养的菌株特征和鼠疫噬菌体裂解试验结果。结果动物实验前鼠疫菌90017在固体培养基上28℃培养24 h时生长缓慢,培养48~100 h后细菌菌落呈典型鼠疫菌形态;经鼠疫噬菌体裂解试验28℃裂解24 h,噬菌带中央呈碎玻璃状、不典型菌落碎片残留,培养48~100 h后鼠疫诊断噬菌体滴下及流过的区域除了受侵蚀的残余鼠疫菌落外,还有个别较完整的鼠疫菌落生长,而对照鼠疫菌141划线区域有明显菌苔,鼠疫诊断噬菌体滴下及流过的区域无细菌生长,形成的噬菌带宽于噬菌体流过的痕迹,鼠疫菌90017经动物实验后未恢复到鼠疫菌典型状态,与动物实验前菌株生长状态和鼠疫噬菌体裂解试验结果相似。结论田鼠型鼠疫菌90017中存在对鼠疫诊断噬菌体具敏感性菌株和抗性菌株共生的现象,这种混合群体的菌株显示了自然环境中微生物群落的生物复杂性,可能在维持鼠疫流行和传播过程中具有重要作用。Objective To analyze the phenotype characteristics of Yersinia pestis Microtus strain 90017 from Lasiopodomys brandtii,so as to provide a theoretical basis for investigating mechanisms of bacterial resistance to phages,the long-term preservation mechanisms of Y.pestis in plague foci,and evolutionary epidemiology of infectious diseases.Methods BALB/c mice were infected subcutaneously with 3 concentrations of Microtus strain of Y.pestis respectively including 2×10^(9),1×10^(6) and 1×10^(5) pieces/ml.Animals are dissected after death to isolated Y.pestis.It were observed on cultural characteristics and plague phage lytic test of Y.pestis Microtus strain 90017.Result Y.pestis 90017 was cultured in a solid medium at 28℃ for 24 hours before and after animal experiment,and it was found that the bacterial growth was slow.The bacterial colony showed typical morphology of Y.pestis after 48-100 hours of incubation.After a 24-hour bacteria lysed at 28℃ in plague phage lysis test,the center of phage lysis zone demonstrated the appearance of broken glass and untypical debris of colonies.After 48-100 hours of incubation,in addition to the eroded residual colonies,some relatively complete colonies remained in the test zone where plague phage dripped and flowed through.The areas marked with the control Y.pestis strain 141 showed obvious bacterial lawn,and there was no bacterial growth in the areas where plague phage dripped and flowed through.The resulting phage lysis zone was wider than the trace left by the phage flow.The bacterium 90017 did not recover to its typical sta te after animal experiment,which was similar to its growth state and the result of plague phage lysis test for plague before animal experiment.Conclusion The coexistence of plague diagnostic bacteriophage-sensitive and-resistant strains in Microtus strain of Y.pestis 90017 from L.brandtii reflects biological complexity of microbial community in natural environment,which may play an important role in maintaining the epidemic and infection process of pest

关 键 词：鼠疫菌 田鼠株 鼠疫诊断噬菌体 抗性 动物实验 布氏田鼠 

分 类 号：R516.8[医药卫生—内科学]